Recurrent chromosomal abnormalities in human embryonic stem cells
Recurrent chromosomal abnormalities in human embryonic stem cells
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DOI：
10.1038/nbt.1510
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发表时间：
2008-12-01
影响因子：
46.9
通讯作者：
Sermon, Karen
中科院分区：
文献类型：
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作者：
Spits, Claudia;Mateizel, Ileana;Sermon, Karen
培养的人胚胎干细胞（hES）具有已知的染色体12、17和X的非整倍体倾向。我们通过基于阵列的比较基因组杂交（aCGH）研究了17个hES细胞系，发现细胞积累了其他复发性染色体异常，包括20q11.21扩增和衍生染色体18。这些基因组变化在转录水平上具有可变的影响。
Cultured human embryonic stem (hES) cells have a known predisposition to aneuploidy of chromosomes 12, 17 and X. We studied 17 hES cell lines by array-based comparative genomic hybridization (aCGH) and found that the cells accumulate other recurrent chromosomal abnormalities, including amplification at 20q11.21 and a derivative chromosome 18. These genomic changes have a variable impact at the transcriptional level.