A simple gradient equilibrium method for better separation in countercurrent chromatography
A simple gradient equilibrium method for better separation in countercurrent chromatography
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一种简单的梯度平衡方法，可在逆流色谱中实现更好的分离
DOI：
10.1002/jssc.201800533
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发表时间：
2018-10-01
影响因子：
3.1
通讯作者：
Di，Duo-Long
中科院分区：
文献类型：
--
作者：
Quan，Kai-Jun;Xi，Xing-jun;Di，Duo-Long
固定相保留值是逆流色谱中最重要的参数之一。本文在传统平衡模型优化条件的基础上，开发了一种简单的梯度平衡方法，以进一步提高固定相保留率。同时，将该方法应用于三种黄酮类模型化合物的分离，并与传统的等度平衡法进行了比较。结果表明，与等度平衡法相比，梯度平衡法可获得更好的分离度或更短的分离时间。因此，这一新的平衡方法在目标化合物的制备分离中获得更好的分离或节省分离时间方面具有巨大的潜力。
The stationary phase retention is one of the most important parameters in countercurrent chromatography. In this work, a simple gradient equilibrium method was developed to further improve the stationary phase retention based on the optimized condition in the traditional equilibrium model. Meanwhile, this novel gradient equilibrium method was used to separate three flavone model compounds and compared with the conventional isocratic equilibrium method to evaluate the separation efficiency. The results show that better resolution or shorter separation time could be achieved with gradient equilibrium compared to isocratic equilibrium. So this novel equilibrium method has enormous potential for obtaining a better separation or saving the separating time in the preparative separation of target compounds.