MEASUREMENT OF LEUCINE METABOLISM IN MAN FROM A PRIMED, CONTINUOUS INFUSION OF L-[1-C-13]LEUCINE
MEASUREMENT OF LEUCINE METABOLISM IN MAN FROM A PRIMED, CONTINUOUS INFUSION OF L-[1-C-13]LEUCINE
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DOI：
10.1152/ajpendo.1980.238.5.e473
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发表时间：
1980-01-01
影响因子：
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通讯作者：
BIER, DM
中科院分区：
文献类型：
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作者：
MATTHEWS, DE;MOTIL, KJ;BIER, DM
亮氨酸体内代谢可以通过在同位素稳态下测量血浆[1 - 13 C]亮氨酸富集、呼出的13 CO2富集和CO2产生速率，从预充的、连续输注的L-[1- 13 C]亮氨酸确定。在适当的L-[1- 13 C]亮氨酸和NaH 13 CO 3预充剂量下，在不到2 h内达到同位素稳态，并在4 h内完成输注。该方法可以确定率亮氨酸营业额，氧化，并纳入蛋白质的典型相对不确定度分别为2%，10%和4%。该方法需要不超过1毫升的血液，并使用稳定同位素而不是放射性同位素技术。该方法适用于所有年龄段的人类研究。L-[1- 13 C]亮氨酸可以与用15 N标记的第二个氨基酸一起注入，用于同时测定2种氨基酸的动力学。
Leucine metabolism in vivo can be determined from a primed, continuous infusion of L-[1-13C]leucine by measuring, at isotopic steady state, plasma [1-13C]leucine enrichment, expired 13CO2 enrichment and CO2 production rate. With an appropriate priming dose of L-[1-13C]leucine and NaH13CO3, isotopic steady state is reached in less than 2 h, and infusion is completed in 4 h. The method can determine rates of leucine turnover, oxidation, and incorporation into protein with typical relative uncertainties of 2, 10 and 4%, respectively. The method requires no more than 1 ml of blood and uses stable isotope rather than radioisotope techniques. The method is applicable to studies of human beings of all ages. L-[1-13C]leucine may be infused with a 2nd amino acid labeled with 15N for simultaneous determination of the kinetics of 2 amino acids.