Collaborative-Tick Surveillance Works: An Academic and Government Partnership for Tick Surveillance in the Southeastern United States (Acari: Ixodidae)
Collaborative-Tick Surveillance Works: An Academic and Government Partnership for Tick Surveillance in the Southeastern United States (Acari: Ixodidae)
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DOI：
10.1093/jme/tjz055
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发表时间：
2019-09-01
影响因子：
2.1
通讯作者：
Vogt, J. T.
中科院分区：
文献类型：
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作者：
Fryxell, R. T. Trout;Vogt, J. T.
壁虱监测提供有关分布和接触频率的基本信息；它是公共卫生实践中业务活动的一个组成部分。我们的研究目标是对协作监测进行概念验证，其中包括建立学术和政府合作伙伴关系，以加强扁虱监测工作。田纳西大学(UT)与美国农业部林业局、南方研究站森林调查分析中心(FIA)合作，建立了职业健康和安全伙伴关系。UT向美国东南部的FIA工作人员提供了含有80%乙醇的瓶子(2014年7月至2017年11月)。机组人员接到指示，将遇到的所有扁虱都放入瓶子里，并将它们送回国际汽联总部。UT鉴定了所有提交的硬蜱的种类和生活阶段，并对Amblyomma americanum(L.)用套式聚合酶链式反应检测埃立克氏菌。从198个回收的试管中，共收集到1180只硬蜱，包括美洲硬蜱(90.51%；202头幼虫、503头若虫和363头成虫)、变色粉虱(7.12%；1头若虫、83头成虫)、肩部硬蜱(1.61%；19头成虫)、斑点钝胸大刺蚁(0.59%；1头若虫、6头成虫)和卡氏钝刺茧蜂(0.17%；1头若虫、1头成虫)。FIA工作人员遇到了感染埃利希菌的美洲钩端螺旋体，收集的信息被用来生成扁虱接触的基线发生数据。结果表明，这种协作-扁虱监测可以得到改进，并用于产生有用的数据，包括病原体检测，并且由于工作人员重新访问这些地点，可以监测扁虱接触的变化。
Tick surveillance provides essential information on distributions and encounter frequencies; it is a component of operational activities in public health practice. Our research objectives were a proof-of-concept for collaborative surveillance, which involved establishing an academic and government partnership to enhance tick surveillance efforts. The University of Tennessee (UT) collaborated with United States Department of Agriculture Forest Service, Southern Research Station Forest Inventory Analysis (FIA) in an Occupational Health and Safety partnership. UT provided FIA crews in the southeastern United States with vials containing 80% ethanol (July 2014-November 2017). Crew members were instructed to put all encountered ticks into the vials and return them to FIA headquarters. UT identified all submitted ticks to species and life stage, and screened Amblyomma americanum (L.) for Ehrlichia bacteria using a nested-PCR assay. From the 198 returned vials, 1,180 ticks were submitted, including A. americanum (90.51%; 202 larvae, 503 nymphs, and 363 adults), Dermacentor variabilis Say (7.12%; 1 nymph, 83 adults), Ixodes scapularis (Say) (1.61%; 19 adults), Amblyomma maculatum Koch (0.59%; 1 nymph, 6 adults), and Amblyomma cajennense (Fabricius) (0.17%; 1 nymph, 1 adult). FIA crews encountered A. americanum with Ehrlichia and collection information was used to generate baseline occurrence data of tick encounters. Results indicate that this collaborative-tick surveillance can be improved and used to generate useful data including pathogen detection, and because crews revisit these sites, changes in tick encounters can be monitored.