Cytoplasmic adenylylation and processing of maternal RNA.
Cytoplasmic adenylylation and processing of maternal RNA.
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细胞质腺苷酸化和母体 RNA 的加工。
DOI：
10.1073/pnas.70.2.406
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发表时间：
1973
影响因子：
11.1
通讯作者：
D. W. Slater
中科院分区：
文献类型：
--
作者：
I. Slater;D. Gillespie;D. W. Slater
[（3）H]-poly（U）和从海胆卵和胚胎中制备的未标记RNA之间的分子杂交已被用于对比未受精卵中预先存在的腺苷酰化母体RNA的亚细胞定位以及大小分布与作为受精功能的腺苷酰化。有证据表明，这种preadenylated的遗传信息主要位于卵子的subribosomal馏分和受精elaries这些潜在的转录到受精卵的核糖体馏分的快速重新分配。对腺苷酰化RNA大小分布的检查进一步证明，未受精卵含有大量分子量极高的RNA转录物，这些转录物用作受精后聚腺苷酸2倍净合成的引物。富含poly（A）的束显示与RNA共价键合。对细胞核和细胞质组分中poly（A）含量的评估表明，poly（A）的功能不仅限于从细胞核转运遗传信息。
Molecular hybridization between [(3)H]-poly(U) and unlabeled RNA prepared from sea urchin eggs and embryos has been used to contrast the subcellular localization as well as the size distribution of adenylylated maternal RNA preexisting in the unfertilized egg with that adenylylated as a function of fertilization. Evidence reported establishes that such preadenylylated genetic messages are predominantly located in the ovum's subribosomal fraction and that fertilization elicits a rapid reallocation of these latent transcripts into the zygote's ribosomal fraction. Examination of the size distribution of the adenylylated RNA further demonstrates that the unfertilized egg contains a substantial population of RNA transcripts of exceptionally high molecular weight that are used as primers for the 2-fold net synthesis of poly(A) that follows fertilization. The poly(A)-rich tracts are shown to be covalently bonded to RNA. Assessment of the poly(A) content of nuclear and cytoplasmic fractions suggests that the function of poly(A) is not confined to the transport of genetic messages from the nucleus.