Characterization of natural frequencies from nanoscale tissue oscillations using dynamic optical coherence elastography
Characterization of natural frequencies from nanoscale tissue oscillations using dynamic optical coherence elastography
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DOI：
10.1364/boe.391324
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发表时间：
2020-06-01
影响因子：
3.4
通讯作者：
Twa, Michael D.
中科院分区：
文献类型：
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作者：
Lan, Gongpu;Larin, Kirill, V;Twa, Michael D.
我们演示了基于 OCT 的弹性成像利用自然频率振荡进行软组织表征。通过垂直空气脉冲刺激在体内组织模型和人角膜中诱导亚微米到亚压力计的振荡，并通过共路径 OCT 成像（灵敏度：0.24 nm）进行观察。使用单自由度方法在时域和频域中获得固有频率和阻尼比。对于不同的刺激压力（4-32 Pa）和测量距离（0.3-5.3 mm），主导固有频率是恒定的，并且随着样品厚度的增加而降低。 0.75-2% 琼脂模型的主要自然频率为 127-774 Hz（平均变异系数 [CV]：0.9%），并与杨氏模量的平方根相关（16.5-117.8 kPa，平均 CV：5.8%）。这些初步研究显示可重复的体内角膜固有频率测量（259 Hz，CV：1.9%）。这种新颖的OCE方法可以利用小幅度组织振荡特征来区分具有不同机械性能的组织和材料，并且适合表征体内脆弱的组织（例如眼睛）。 (C) 2020 年美国光学学会根据 OSA 开放获取出版协议的条款
We demonstrate the use of OCT-based elastography for soft-tissue characterization using natural frequency oscillations. Sub-micrometer to sub-manometer oscillations were induced in tissue phantoms and human cornea in vivo by perpendicular air-pulse stimulation and observed by common-path OCT imaging (sensitivity: 0.24 nm). Natural frequency and damping ratio were acquired in temporal and frequency domains using a single degree of freedom method. The dominant natural frequency was constant for different stimulation pressures (4-32 Pa) and measured distances (0.3-5.3 mm), and decreased as the sample thickness increased. The dominant natural frequencies of 0.75-2% agar phantoms were 127-774 Hz (mean coefficient of variation [CV]: 0.9%), and correlated with the square root of Young's moduli (16.5-117.8 kPa, mean CV: 5.8%). These preliminary studies show repeatable in vivo corneal natural frequency measurements (259 Hz, CV: 1.9%). This novel OCE approach can distinguish tissues and materials with different mechanical properties using the small-amplitude tissue oscillation features, and is suitable for characterizing delicate tissues in vivo such as the eye. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement