The Long Noncoding RNA LINC00908 Facilitates Hepatocellular Carcinoma Progression Via Interaction With Sox-4 (Retracted Article)
The Long Noncoding RNA LINC00908 Facilitates Hepatocellular Carcinoma Progression Via Interaction With Sox-4 (Retracted Article)
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DOI：
10.2147/cmar.s216774
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发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Zhang, Jinfeng
中科院分区：
文献类型：
--
作者：
Hu, Xinhua;Li, Qingxiang;Zhang, Jinfeng
背景：肝细胞癌（HCC）是世界范围内一种高度侵袭性且常见的恶性肿瘤。越来越多的证据表明长链非编码 RNA (lncRNA) 在各种肿瘤中发挥着关键作用。然而，基因间lncRNA LINC00908在HCC中的功能仍不清楚。方法：采用RT-qPCR方法定量LINC00908的表达。进行迁移和活力测定来评估体外效果，并使用异种移植肿瘤模型来测量体内效果。使用免疫印迹来鉴定LINC00908与Sox-4的关联以及Sox-4的稳定性。结果：我们发现了与HCC相关的新型lncRNA。与正常组织和细胞系相比，LINC00908 在肿瘤组织和细胞系中高表达。 LINC00908 高表达与晚期 TNM 分期、肿瘤大小和转移相关。 LINC00908 促进 HCC 细胞的迁移和活力。 LINC00908过表达也增强了体内异种移植肿瘤的生长和增殖，而LINC00908沉默则抑制了体内异种移植肿瘤的生长和增殖。 LINC00908 与 Sox-4 发生物理相互作用，LINC00908 和 Sox-4 之间的关联通过减少蛋白酶体降解来增加 Sox-4 的稳定性。结论：综上所述，我们目前的工作已经鉴定出一种新的 lncRNA LINC00908，它在 HCC 进展中发挥着关键作用。 LINC00908-Sox-4 轴可能为药物治疗提供新的潜在靶点。
Background: The hepatocellular carcinoma (HCC) is a highly aggressive and common malignancy worldwide. Accumulating evidence has demonstrated a pivotal role of long noncoding RNAs (lncRNAs) in various tumors. However, the function of intergenic lncRNA LINC00908 is still unknown in HCC.Methods: The RT-qPCR method was used to quantify the expression of LINC00908. Migration and viability assay were performed to evaluate the in vitro effect and xenograft tumor model was used to measure the in vivo effect Immunoblot was used to identify the association of LINC00908 with Sox-4 and the stability of Sox-4.Results: We found a novel lncRNA related to HCC. LINC00908 is highly expressed in tumorous tissues and cell lines compared with normal ones. High LINC00908 expression correlated with advanced TNM stages, tumor size and metastasis. LINC00908 promoted the migration and viability of HCC cells. The in vivo xenograft tumor growth and proliferation were also enhanced by LINC00908 overexpression and inhibited by LINC00908 silence. LINC00908 physically interacted with Sox-4, and the association between LINC00908 and Sox-4 increased the stability of Sox-4 by reducing proteasomal degradation.Conclusion: Taken together, our current work has identified a novel lncRNA LINC00908 which is critically involved in HCC progression. The LINC00908-Sox-4 axis might provide a new and potential target for pharmaceutical therapies.