Vitamin A deficiency alters the pulmonary parenchymal elastic modulus and elastic fiber concentration in rats.
Vitamin A deficiency alters the pulmonary parenchymal elastic modulus and elastic fiber concentration in rats.
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DOI：
10.1186/1465-9921-6-77
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发表时间：
2005-07-20
影响因子：
5.8
通讯作者：
Holmes AJ
中科院分区：
文献类型：
--
作者：
McGowan SE;Takle EJ;Holmes AJ
Bronchial hyperreactivity is influenced by properties of the conducting airways and the surrounding pulmonary parenchyma, which is tethered to the conducting airways. Vitamin A deficiency (VAD) is associated with an increase in airway hyperreactivity in rats and a decrease in the volume density of alveoli and alveolar ducts. To better define the effects of VAD on the mechanical properties of the pulmonary parenchyma, we have studied the elastic modulus, elastic fibers and elastin gene-expression in rats with VAD, which were supplemented with retinoic acid (RA) or remained unsupplemented. Parenchymal mechanics were assessed before and after the administration of carbamylcholine (CCh) by determining the bulk and shear moduli of lungs that that had been removed from rats which were vitamin A deficient or received a control diet. Elastin mRNA and insoluble elastin were quantified and elastic fibers were enumerated using morphometric methods. Additional morphometric studies were performed to assess airway contraction and alveolar distortion. VAD produced an approximately 2-fold augmentation in the CCh-mediated increase of the bulk modulus and a significant dampening of the increase in shear modulus after CCh, compared to vitamin A sufficient (VAS) rats. RA-supplementation for up to 21 days did not reverse the effects of VAD on the elastic modulus. VAD was also associated with a decrease in the concentration of parenchymal elastic fibers, which was restored and was accompanied by an increase in tropoelastin mRNA after 12 days of RA-treatment. Lung elastin, which was resistant to 0.1 N NaOH at 98°, decreased in VAD and was not restored after 21 days of RA-treatment. Alterations in parenchymal mechanics and structure contribute to bronchial hyperreactivity in VAD but they are not reversed by RA-treatment, in contrast to the VAD-related alterations in the airways.
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DOI：
10.1016/0034-5687(80)90107-3
发表时间：
1980-01-01
期刊：
RESPIRATION PHYSIOLOGY
影响因子：
--
作者：
SCHROTER, RC
通讯作者：
SCHROTER, RC
DOI：
10.1165/ajrcmb.23.2.3904
发表时间：
2000-08-01
影响因子：
6.4
作者：
McGowan, S;Jackson, SK;Snyder, JM
通讯作者：
Snyder, JM
DOI：
10.1152/ajplung.00319.2001
发表时间：
2002-05-01
影响因子：
4.9
作者：
McGowan, SE;Smith, J;Kline, JN
通讯作者：
Kline, JN
DOI：
10.1164/ajrccm/140.4.1067
发表时间：
1989-10-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
BRUCE, MC;PAWLOWSKI, R;TOMASHEFSKI, JF
通讯作者：
TOMASHEFSKI, JF
DOI：
10.1164/ajrccm/138.1.136
发表时间：
1988-07-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
JAMES, AL;HOGG, JC;PARE, PD
通讯作者：
PARE, PD