Light-triggered explosion of lipid vesicles
Light-triggered explosion of lipid vesicles
复制标题
光触发脂质囊泡爆炸
DOI：
10.1039/d0sm01027h
复制
发表时间：
2020
期刊：
影响因子：
3.4
通讯作者：
Feng, Jie
中科院分区：
文献类型：
--
作者：
Malik, Vinit Kumar;Shin, Sangwoo;Feng, Jie
脂质囊泡已经收到了相当大的兴趣，因为它们的应用，在体外还原细胞膜模型以及治疗传递车辆。在这些情况下，囊泡在非平衡环境中的机械响应在确定相应的动力学中起着关键作用。对脂质囊泡在暴露于低渗溶液时的响应的共同理解是特征性脉动行为。然而，最近的实验表明，囊泡在光反应产生的渗透压冲击下爆炸，但解释机制尚不清楚。在这里，我们提出了一个广义的生物物理模型，将膜破裂的随机帐户来描述膨胀-爆裂-再密封循环和爆炸动力学。该模型与实验观察结果吻合良好，并且它揭示了突然的渗透冲击以极端的速率使囊泡应变，将囊泡驱动到屈曲不稳定性中，从而导致膜破裂，即爆炸。我们的工作不仅推进了渗透压下的非平衡囊泡动力学的基本框架，但也提供了可编程囊泡封装的物质释放在治疗载体的设计准则。
Lipid vesicles have received considerable interest because of their applications to in vitro reductionist cell membrane models as well as therapeutic delivery vehicles. In these contexts, the mechanical response of vesicles in nonequilibrium environments plays a key role in determining the corresponding dynamics. A common understanding of the response of lipid vesicles upon exposure to a hypotonic solution is a characteristic pulsatile behavior. Recent experiments, however, have shown vesicles exploding under an osmotic shock generated by photo-reactions, yet the explanatory mechanism is unknown. Here we present a generalized biophysical model incorporating a stochastic account of membrane rupture to describe both swell-burst-reseal cycling and exploding dynamics. This model agrees well with experimental observations, and it unravels that the sudden osmotic shock strains the vesicle at an extreme rate, driving the vesicle into buckling instabilities responsible for membrane fragmentation, i.e. explosion. Our work not only advances the fundamental framework for non-equilibrium vesicle dynamics under osmotic stress, but also offers design guidelines for programmable vesicle-encapsulated substance release in therapeutic carriers.