A portable spinning disc for complete blood count (CBC)
A portable spinning disc for complete blood count (CBC)
复制标题
DOI：
10.1016/j.bios.2019.111935
复制
发表时间：
2020-02-15
影响因子：
12.6
通讯作者：
Chakraborty, Suman
中科院分区：
文献类型：
--
作者：
Agarwal, Rahul;Sarkar, Arnab;Chakraborty, Suman
全血细胞计数（CBC）是最常用的临床测试的集合，以评估血液病理状况的表现。由于需要复杂的仪器和训练有素的人员，现有的临床方法对于全球贫困人口来说过于昂贵。为了克服这些问题，我们提出了一种独特的低成本设备作为血细胞计数平台。该方法利用透明微流控通道中细胞密度的差异进行分离，实现了无标记成像方法对微流控盘内分离细胞计数。该设备是一个简单的旋转圆盘，用于估计红细胞压积、血红蛋白、红细胞（RBC）、白细胞（WBC）和血小板计数等参数，与自动血液学分析仪相比，准确度为95%。该设备相对于现有技术的主要优点包括在单个可生物降解的圆盘内进行多个样品测试，简单的设计和制造技术，潜在的自动化，从而使其便携，不需要训练有素的人员，最重要的是，不需要对分离的血液进行下游处理。这些结果可能会被证明是巨大的后果对开发新的点护理血液分析仪资源有限的设置。
Complete Blood Count (CBC) is a collection of the most commonly required clinical tests to assess the manifestations of pathological conditions in blood. The existing clinical methods for this test are prohibitively expensive for the underprivileged global population due to the requirements of sophisticated instrumentation and trained personnel. To overcome these, we propose a unique low cost device as a blood cell counting platform. The method exploits the difference in densities of cells for separation in transparent microfluidic channels and implements label-free imaging method for counting the separated cells within the microfluidic disc. The device is a simple spinning disc to estimate the parameters such as hematocrit, hemoglobin, red blood cell (RBC), white blood cell (WBC), and platelet counts with an accuracy > 95% as compared to an automated hematology analyzer. The major advantages of this device over state of the art include multiple sample testing within a single biodegradable disc, simple design and fabrication techniques, potential automation thereby making it portable and eliminating the need of trained personnel, and most significantly, eliminating any need for downstream processing of the separated blood. These results may turn out to be of immense consequence towards developing novel point-of-care hematological analyzers for resource-constrained settings.