The mu opioid receptor activation does not affect ischemia-induced agonal currents in rat spinal ventral horn.
The mu opioid receptor activation does not affect ischemia-induced agonal currents in rat spinal ventral horn.
复制标题
μ阿片受体激活不影响大鼠脊髓腹角缺血诱导的激动电流。
DOI：
10.1007/s00540-014-1829-3
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发表时间：
2014
影响因子：
2.8
通讯作者：
Tatsuro Kohno
中科院分区：
文献类型：
--
作者：
Hiroyuki Honda;Hiroshi Baba;Tatsuro Kohno
目的：已有文献报道，在主动脉手术中，阿片类药物引起的脊髓短暂缺血后痉挛性截瘫。阿片类物质通过脊髓腹角中的μ阿片受体（MORs）调节神经传递。方法采用全细胞膜片钳技术，观察μ受体激动剂[d-Ala 2，-N-Me-Phe 4，Gly 5-ol]脑啡肽（DAMGO）对新生大鼠脊髓IX板层神经元缺血性濒死电流的影响。结果DAMGO（1 μM）在维持电位为−70 mV时，在~ 60%的脊髓IX层神经元中产生外向电流。用模拟缺血介质灌流引起了一个濒死电流。潜伏期为457 ± 18 s。尽管DAMGO具有神经调节作用，但在有（440 ± 23 s）或无（454 ± 33 s）DAMGO诱导电流的情况下，DAMGO均不显著改变濒死电流的延迟时间。我们的研究结果表明，莫尔激动剂管理应适合作为麻醉主动脉手术。
PurposeOpioid-induced spastic paraplegia after transient spinal cord ischemia during aortic surgery has been reported. Opioids modulate neurotransmission through mu (μ) opioid receptors (MORs) in the spinal ventral horn. However, their effects during ischemic insult are not understood.MethodsThe effects of the selective μ agonist [d-Ala2,-N-Me-Phe4, Gly5-ol]enkephalin (DAMGO) on ischemia-induced agonal currents were examined in the spinal lamina IX neurons of neonatal rats by using the whole-cell patch-clamp technique. Ischemia was simulated in vitro by oxygen/glucose deprivation.ResultsDAMGO (1 μM) produced outward currents in ~60 % of spinal lamina IX neurons at a holding potential of −70 mV. Superfusion with ischemia-simulating medium elicited an agonal current. The latency was 457 ± 18 s. Despite its neuromodulatory effects, DAMGO did not significantly change the latencies of the agonal currents with (440 ± 23 s) or without (454 ± 33 s) DAMGO-induced currents.ConclusionActivation of MORs does not influence ongoing ischemia-induced neuronal death. Our findings indicate that MOR agonist administration should be suitable as an anesthetic during aortic surgery.