Identification of a redox-dependent regulatory network of miRNAs and their targets in wheat
Identification of a redox-dependent regulatory network of miRNAs and their targets in wheat
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DOI：
10.1093/jxb/ery339
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发表时间：
2019-01-01
影响因子：
6.9
通讯作者：
Kocsy, Gabor
中科院分区：
文献类型：
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作者：
Cao, Jie;Gulyas, Zsolt;Kocsy, Gabor
活性氧和抗氧化剂在调节植物生长发育中具有重要作用。在这项研究中，我们研究了小麦（Triticum aestivum ssp. aestivum）。10 mM H2 O2通过根处理幼苗24 h，导致谷胱甘肽含量降低，谷胱甘肽二硫化物/谷胱甘肽氧化还原对的半细胞还原电位增加，和更大的抗坏血酸过氧化物酶活性相比，对照植物。这些变化伴随着miRNA转录谱的改变，其中70种miRNA在对照和处理（0、3、6 h）幼苗之间的表达差异至少为1.5倍。Degradome测序鉴定了这些miRNAs的86个靶基因，并使用生物信息学工具鉴定了6722个可能的额外靶基因。转录组分析也证实了1647个靶基因在处理24 h后的H2 O2响应性，发现它们主要与氧化还原过程、转录、蛋白质磷酸化和降解的控制有关。在时间过程实验中（处理0-24小时），发现谷胱甘肽、其他抗氧化剂的水平与H2 O2响应性miRNA及其靶mRNA的转录水平之间存在相关性。这种关系与调控网络的生物信息学建模一起表明了谷胱甘肽相关的miRNA及其靶点的氧化还原控制，这使得代谢能够根据不断变化的环境条件进行调整。
Reactive oxygen species and antioxidants have an important role in the regulation of plant growth and development under both optimal and stress conditions. In this study, we investigate a possible redox control of miRNAs in wheat (Triticum aestivum ssp. aestivum). Treatment of seedlings with 10 mM H2O2 via the roots for 24 h resulted in decreased glutathione content, increased half-cell reduction potential of the glutathione disulphide/glutathione redox pair, and greater ascorbate peroxidase activity compared to the control plants. These changes were accompanied by alterations in the miRNA transcript profile, with 70 miRNAs being identified with at least 1.5-fold difference in their expression between control and treated (0, 3, 6 h) seedlings. Degradome sequencing identified 86 target genes of these miRNAs, and 6722 possible additional target genes were identified using bioinformatics tools. The H2O2-responsiveness of 1647 target genes over 24 h of treatment was also confirmed by transcriptome analysis, and they were mainly found to be related to the control of redox processes, transcription, and protein phosphorylation and degradation. In a time-course experiment (0-24 h of treatment) a correlation was found between the levels of glutathione, other antioxidants, and the transcript levels of the H2O2-responsive miRNAs and their target mRNAs. This relationship together with bioinformatics modelling of the regulatory network indicated glutathione-related redox control of miRNAs and their targets, which allows the adjustment of the metabolism to changing environmental conditions.