Unique quenching of fluorescent copper nanoclusters based on target-induced oxidation effect: a simple, label-free, highly sensitive and specific bleomycin assay
Unique quenching of fluorescent copper nanoclusters based on target-induced oxidation effect: a simple, label-free, highly sensitive and specific bleomycin assay
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DOI：
10.1039/c6ra09054k
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Li, Feng
中科院分区：
文献类型：
--
作者：
Li, Haiyin;Wang, Chuanfeng;Li, Feng
在这方面的贡献，一种新的无标记的荧光生物传感器博莱霉素（BLM）检测相结合的铜纳米团簇（CuNCs）和独特的氧化能力的BLM-Fe 2+络合物对CuNCs。以单链DNA为模板，通过原位形成CuNCs，制备了CuNCs探针，使探针具有良好的亲水性，这对生物样品的分析具有重要意义。在它们识别BLM后，CuNCs被破坏，探针的红色荧光被淬灭，从而实现对BLM的检测。这种荧光传感策略允许高灵敏度的BLM生物传感，检测限低至0.26 nM，复杂混合物的干扰最小。与以往报道的方法相比，该方法不需要特定的DNA序列、复杂的设计和信号分子标记，避免了繁琐的实验步骤，具有简单、成本低的优点。此外，我们的探针也被用于检测BLM在人类血清样品中，并取得了优异的性能，这使得所提出的策略的一个有前途的候选人的BLM在癌症治疗中的高灵敏度和特异性的分析。
In this contribution, a novel label-free fluorescence biosensor for bleomycin (BLM) detection was developed by combining the excellent fluorescence properties of copper nanoclusters (CuNCs) and the unique oxidation capability of a BLM-Fe2+ complex toward CuNCs. The CuNCs probe was prepared through in situ formation of CuNCs using single-stranded DNAs as the templates, endowing the probe with good water-dispersibility that is important for analyzing biological samples. After their recognition of BLM, the CuNCs were destroyed and the red fluorescence of the probe was quenched, thus realizing the detection of BLM. Such a fluorescence sensing strategy allows for highly sensitive BLM biosensing with a detection limit as low as 0.26 nM and minimal interference from complex mixtures. Compared to previously reported methods, the as-proposed assay does not need specific DNA sequences, complex designing or signal molecule labeling, and further avoids tedious experimental procedures, thus providing the strategy with additional advantages of simplicity and cost-effectiveness. Furthermore, our probe was also adopted for the detection of BLM in human serum samples and excellent performance was achieved, which makes the as-proposed strategy a promising candidate for highly sensitive and specific analysis of BLM in cancer treatment.