Occurrence of purulent arthritis broilers vertically infected with Salmonella enterica serovar Enteritidis in Korea
Occurrence of purulent arthritis broilers vertically infected with Salmonella enterica serovar Enteritidis in Korea
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DOI：
10.3382/ps.2010-00918
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发表时间：
2010-10-01
期刊：
影响因子：
4.4
通讯作者：
Kwon, Y-K.
中科院分区：
文献类型：
--
作者：
Oh, J-Y.;Kang, M-S.;Kwon, Y-K.
肠道沙门氏菌（Salmonella enterica serovar Enterilis，简称沙门氏菌）与世界范围内肉鸡的发病率和死亡率有关。本研究描述了感染肠炎沙门氏菌的肉鸡化脓性关节炎，并调查了流行病学相关的财产，如孵化场和种鸡场，试图阐明污染源的肠炎沙门氏菌分离株的基因图谱和遗传特征。在受影响的肉鸡群中观察临床疾病和死亡率。至12日龄死亡率为5.8%。这些鸟通常表现出跛行，飞节和足垫中度肿胀。最常见的病变是严重化脓性关节炎伴多浆膜炎。组织学检查显示腿部关节和内脏器官的滑膜中有中度至重度炎症。当使用18种抗菌药物通过纸片扩散法对来自肉鸡和相关孵化场和种鸡场的7株肠道沙门氏菌分离株进行抗菌药物敏感性试验时，来自肉鸡和种鸡场的分离株具有相同的细菌谱，其特征在于对氨苄青霉素、头孢噻呋、头孢噻吩、庆大霉素、萘啶酸、链霉素、磺胺异恶唑和四环素的多重耐药性，而来自孵化场的分离株仅对萘啶酸具有不同的抗性。通过使用限制性内切酶XbaI的脉冲场凝胶电泳的遗传分析，来自肉鸡和种鸡场的肠道沙门氏菌分离株与耐萘啶酸的孵化场分离株相比也显示出相同的PFGE模式。因此，从肉鸡和种鸡场分离的沙门氏菌之间相同的PFGE谱和电泳图谱提供了直接的证据，从污染的种鸡场到商业肉鸡的肠道沙门氏菌垂直传播。
Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) has been associated with morbidity and mortality in broiler chickens worldwide. The present study described purulent arthritis of broilers infected with Salmonella Enteritidis and investigated antibiograms and genetic characteristics of Salmonella Enteritidis isolates from epidemiologically related properties such as a hatchery and breeder farm in an attempt to elucidate the source of contamination. Clinical disease and mortality were observed in the affected broiler flock. Mortality was 5.8% until 12 d of age. The birds typically showed lameness with moderately swollen hock joints and footpads. The most prevalent lesions were severely purulent arthritis with polyserositis. Histopathology revealed moderate to severe inflammation in the synovial membrane of leg joints and visceral organs. When the antimicrobial susceptibility test was performed against 7 isolates of Salmonella Enteritidis from broilers, and relevant hatchery and breeder farms by the disk diffusion method using 18 antimicrobial agents, isolates from broiler and breeder farms had the same antibiogram characterized by multiple drug resistance to ampicillin, ceftiofur, cephalothin, gentamycin, nalidixic acid, streptomycin, sulfisoxazole, and tetracycline, whereas isolates from the hatchery were differently resistant to only nalidixic acid. Through the genetic analysis with pulsed-field gel electrophoresis using the restriction enzyme XbaI, Salmonella Enteritidis isolates from both broiler and breeder farms also showed the same PFGE pattern compared with the hatchery isolates resistant to nalidixic acid. As a result, the same PFGE profiles and antibiogram patterns among isolates from broilers and breeder farms provided direct evidence of vertical Salmonella Enteritidis transmission from the contaminated breeder farm to commercial broiler.