Adipokinetic hormone receptor gene identification and its role in triacylglycerol mobilization and sexual behavior in the oriental fruit fly (Bactrocera dorsalis)
Adipokinetic hormone receptor gene identification and its role in triacylglycerol mobilization and sexual behavior in the oriental fruit fly (Bactrocera dorsalis)
复制标题
脂肪运动激素受体基因鉴定及其在东方果蝇（橘小实蝇）三酰甘油动员和性行为中的作用
DOI：
10.1016/j.ibmb.2017.09.006
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发表时间：
2017-11-01
影响因子：
3.8
通讯作者：
Smagghe, Guy
中科院分区：
文献类型：
--
作者：
Hou, Qiu-Li;Chen, Er-Hu;Smagghe, Guy
能量稳态需要对能量消耗和食物资源供应的波动进行持续补偿。在昆虫中，能量动员是由脂肪动力学激素（AKH）控制的，它通过支持脂质动员来调节营养状况。在本研究中，我们鉴定了AKH受体（AKHR）的基因编码，并研究了其在热带和亚热带水果中最重要的害虫之一东方果蝇（Bactrocera dorsalis）中的功能。Bacdo-AKHR是一种典型的G蛋白偶联受体（GPCR），系统发育分析证实Bacdo-AKHR与其他物种的昆虫AKHRs有密切的亲缘关系。在中国仓鼠卵巢（CHO）细胞中表达后，Bacdo-AKHR对AKH肽具有较高的敏感性和选择性（EC50 = 19.3 nM）。利用qPCR技术，发育阶段和组织特异性表达谱显示，Bacdo-AKHR在幼虫期和成虫期都有高表达，并且在成虫的脂肪体和中肠中也有高表达，性别无差异。为了研究AKHR在背salis中的作用，我们用dsRNA对雌雄成蝇在饥饿和摄食条件下的Bacdo-AKHR进行了RNAi检测。主要结果是，该基因的敲低导致三酰基甘油（TAG）的积累。对于rnai雄性，我们观察到它们在饥饿时的求偶行为严重减少，但在进食时，它们的交配行为有部分恢复。同样在rnai雄性中，与对照组相比，饥饿时系留飞行时间减少，这证实了对能量代谢的依赖。rnai雌虫的性行为不受影响，但生殖力下降。我们的研究结果表明，AKHR在男性的性行为中起着有趣的作用。这种效应与TAG积累有关，我们也报道了akh介导的系统在背芽孢杆菌中的保守作用是营养状态依赖的。因此，我们进一步了解了AKH/AKHR在背孢白僵菌中的多种功能。(C) 2017 Elsevier Ltd.版权所有。
Energy homeostasis requires continuous compensation for fluctuations in energy expenditure and availability of food resources. In insects, energy mobilization is under control of the adipokinetic hormone (AKH) where it is regulating the nutritional status by supporting the mobilization of lipids. In this study, we characterized the gene coding for the AKH receptor (AKHR) and investigated its function in the oriental fruit fly (Bactrocera dorsalis) that is economically one of the most important pest insects of tropical and subtropical fruit. Bacdo-AKHR is a typical G protein-coupled receptor (GPCR) and phylogenetic analysis confirmed that Bacdo-AKHR is closely related to insect AKHRs from other species. When expressed in Chinese hamster ovary (CHO) cells, Bacdo-AKHR exhibited a high sensitivity and selectivity for AKH peptide (EC50 = 19.3 nM). Using qPCR, the developmental stage and tissue-specific expression profiles demonstrated that Bacdo-AKHR was highly expressed in both the larval and adult stages, and also specifically in the fat body and midgut of the adult with no difference in sex. To investigate the role of AKHR in B. dorsalis, RNAi assays were performed with dsRNA against Bacdo-AKHR in adult flies of both sexes and under starvation and feeding condition. As major results, the knockdown of this gene resulted in triacylglycerol (TAG) accumulation. With RNAi-males, we observed a severe decrease in their sexual courtship activity when starved, but there was a partial rescue in copulation when refed. Also in RNAi-males, the tethered-flight duration declined compared with the control group when starved, which is confirming the dependency on energy metabolism. In RNAi-females, the sexual behavior was not affected, but their fecundity was decreased. Our findings indicate an interesting role of AKHR in the sexual behavior of males specifically. The effects are associated with TAG accumulation, and we also reported that the conserved role of AKH-mediated system in B. dorsalis is nutritional state-dependent. Hence, we provided further understanding on the multiple functions of AKH/AKHR in B. dorsalis. (C) 2017 Elsevier Ltd. All rights reserved.