Magnetic Chip Based Extracorporeal Circulation: A New Tool for Circulating Tumor Cell in Vivo Detection
Magnetic Chip Based Extracorporeal Circulation: A New Tool for Circulating Tumor Cell in Vivo Detection
复制标题
基于磁性芯片的体外循环：体内循环肿瘤细胞检测的新工具
DOI：
10.1021/acs.analchem.9b04286
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发表时间：
2019-12-03
影响因子：
7.4
通讯作者：
Zhang, Zhi-Ling
中科院分区：
文献类型：
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作者：
Tang, Man;Xia, Hou-Fu;Zhang, Zhi-Ling
在体内检测循环肿瘤细胞(CTCs)是检测体内所有循环血液的方法，在细胞捕获方面似乎更具优势，特别是在癌症的早期诊断中。因此，基于体内微流控芯片检测系统(IV-CHIP-SYSTEM)，构建了一个体外循环，以有效地检测和监测体内CTCs。结合微流控芯片和免疫磁性纳米球(IMN)，该系统不仅是CTC监测的窗口，也是进一步癌症诊断和CTC研究的收集器。与目前的活体检测方法相比，该系统无需任何预处理即可捕获和检测血液中的CTCs，并且具有更高的CTC捕获效率。值得一提的是，该系统稳定、生物兼容，对活体动物没有任何不可逆转的损害。利用该系统对模拟的CTC在血管中的清除过程进行监测，可能会为癌症研究和癌症早期诊断提供新的见解。
In vivo detection of circulating tumor cells (CTCs) which inspect all of the circulating blood in body seems to have more advantages on cell capture, especially in earlier cancer diagnosis. Herein, based on in vivo microfluidic chip detection system (IV-chip-system), an extracorporeal circulation was constructed to effectively detect and monitor CTCs in vivo. Combined with microfluidic chip and immunomagnetic nanosphere (IMN), this system not only acts as a window for CTC monitoring but also serves as a collector for further cancer diagnosis and research on CTCs. Compared with the current in vivo detection method, this system can capture and detect CTCs in the bloodstream without any pretreatments, and it also has a higher CTC capture efficiency. It is worth mentioning that this system is stable and biocompatible without any irreversible damage to living animals. Taking use of this system, the mimicked CTC cleanup process in the blood vessel is monitored, which may open new insights in cancer research and early cancer diagnosis.