NEUROFILAMENT ANTIBODIES AND SPIRAL GANGLION NEURONS OF THE MAMMALIAN COCHLEA
NEUROFILAMENT ANTIBODIES AND SPIRAL GANGLION NEURONS OF THE MAMMALIAN COCHLEA
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DOI：
10.1002/cne.903060304
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发表时间：
1991-04-15
影响因子：
2.5
通讯作者：
RYUGO, DK
中科院分区：
文献类型：
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作者：
BERGLUND, AM;RYUGO, DK
用各种单克隆神经丝抗体对猫、沙鼠、小鼠、大鼠和人的螺旋神经节进行化学染色。 针对200-kD神经丝蛋白的三种抗体（R-3，Drager等人，'84; ICN抗-200，克隆NE 14，Debus等，'83; RT-97，Wood和安德顿，'81）标记了II型螺旋神经节神经元的胞体，但不标记I型神经节神经元的胞体。 在猫、小鼠和大鼠耳蜗的最底部，有少数（< 0.5%的总神经节群）类似于I型神经节神经元的大神经元的强烈标记。其他几种神经丝抗体（阿默舍姆抗-68、阿默舍姆和ICN抗-160和SMI-32）没有特异性标记II型神经节神经元，而是标记螺旋神经节的所有神经元。 这两种标记模式促使我们调查这种差异的原因。 因为200 kD的神经丝蛋白抗体优先标记II型神经元，因为200 kD的神经丝是高度磷酸化，我们处理耳蜗组织与碱性磷酸酶，以消除磷酸基团。 这种处理消除了用R-3、ICN抗200和RT-97对II型神经元的强烈标记，但对用测试的其他神经丝抗体观察到的神经节细胞体的强烈标记没有影响。 这一证据表明，标签发生，因为磷酸化的200 kD神经丝蛋白在II型神经节神经元的细胞质存在。 因此，神经元群体的神经丝表位可能不同，单克隆抗体可用于标记这种差异。
The spiral ganglia of the cat, gerbil, mouse, rat, and human were immunohistochemically stained with various monoclonal neurofilament antibodies. Three antibodies to the 200-kD neurofilament protein (R-3, Drager et al., '84; ICN anti-200, clone NE14, Debus et al., '83; RT-97, Wood and Anderton, '81) labeled the somata of type II spiral ganglion neurons but not those of type I ganglion neurons. In the extreme base of the cochlea of cats, mice and rats, there was intense labeling of a few (< 0.5% of the total ganglion population) large neurons resembling type I ganglion neurons.Several other neurofilament antibodies (Amersham anti-68, Amersham and ICN anti-160, and SMI-32) did not specifically label type II ganglion neurons but instead labeled all neurons of the spiral ganglion. These two patterns of labeling prompted us to investigate the cause for this difference. Because antibodies against the 200-kD neurofilament protein preferentially labeled type II neurons and because 200-kD neurofilament is highly phosphorylated, we treated cochlear tissue with alkaline phosphatase in order to remove phosphate groups. This treatment eliminated the intense labeling of type II neurons with R-3, ICN anti-200, and RT-97, but had no effect on the intense labeling of ganglion cell bodies observed with the other neurofilament antibodies tested. This evidence suggests that labeling occurs because of the cytoplasmic presence of phosphorylated 200-kD neurofilament protein in type II ganglion neurons. Populations of neurons may thus differ in their neurofilament epitopes and monoclonal antibodies can be used to mark such differences.