Induction of systemic disease resistance in Nicotiana benthamiana by the cyclodipeptides cyclo (l-Pro-l-Pro) and cyclo (d-Pro-d-Pro)
Induction of systemic disease resistance in Nicotiana benthamiana by the cyclodipeptides cyclo (l-Pro-l-Pro) and cyclo (d-Pro-d-Pro)
复制标题
环二肽环（l-Pro-l-Pro）和环（d-Pro-d-Pro）诱导本塞姆氏烟草的全身抗病性
DOI：
10.1111/mpp.12381
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发表时间：
2017
影响因子：
4.9
通讯作者：
Gao Xuewen
中科院分区：
文献类型：
--
作者：
Wu Liming;Wu Huijun;Chen Lina;Zhang Hongyue;Gao Xuewen
环二肽是由两个氨基酸通过环脱水反应形成的，是许多生物天然产生的产物，具有丰富的生物活性。在这项研究中，我们发现环(L-Pro-L-Pro)和环(d-Pro-d-Pro)(其中Pro是Pro)可以诱导烟草的防御反应和系统抗性。与对照相比，用这两种环二肽处理可以降低烟草疫霉和烟草花叶病毒(TMV)感染的病情严重程度。这两种环肽均可触发气孔关闭，诱导保卫细胞产生活性氧，并刺激胞内钙离子和一氧化氮的产生。此外，环二肽的应用显著上调了植物防御基因PR-1a和PR-1a蛋白的表达，并增加了细胞水杨酸(SA)的水平。这些结果表明，SA依赖的防御途径参与了环二肽介导的病原菌抗性。本特米亚纳。我们报道了环二肽诱导的系统抗性，这为环二肽在控制植物病害方面的潜力提供了线索。
Cyclodipeptides, formed from two amino acids by cyclodehydration, are produced naturally by many organisms, and are known to possess a large number of biological activities. In this study, we found that cyclo (l‐Pro‐l‐Pro) and cyclo (d‐Pro‐d‐Pro) (where Pro is proline) could induce defence responses and systemic resistance inNicotiana benthamiana. Treatment with the two cyclodipeptides led to a reduction in disease severity byPhytophthora nicotianaeandTobacco mosaic virus(TMV) infections compared with controls. Both cyclopeptides triggered stomatal closure, induced reactive oxygen species production and stimulated cytosolic calcium ion and nitric oxide production in guard cells. In addition, the application of cyclodipeptides significantly up‐regulated the expression of the plant defence genePR‐1aand the PR‐1a protein, and increased cellular salicylic acid (SA) levels. These results suggest that the SA‐dependent defence pathway is involved in cyclodipeptide‐mediated pathogen resistance inN. benthamiana. We report the systemic resistance induced by cyclodipeptides, which sheds light on the potential of cyclodipeptides for the control of plant diseases.