Influence of coronary flow and pressure on cardiac function and coronary vascular volume.
Influence of coronary flow and pressure on cardiac function and coronary vascular volume.
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冠状动脉流量和压力对心功能和冠状血管容量的影响。
DOI：
10.1152/ajplegacy.1973.224.4.918
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
B. Bromberger
中科院分区：
文献类型：
--
作者：
S. Scharf;B. Bromberger
材料和方法使用一种分离的代谢支持的狗心脏制备物（图1）。先前已经描述了提取心脏并通过气管将其悬挂在Lucite盒中进行灌注的方法（2 - 7）。将来自麻醉（戊巴比妥30 mg/kg iv）支持犬颈动脉的肝素化血液泵入离体心脏的主动脉，其中主动脉瓣确保血液被引导至冠状动脉系统。对冠状窦（CS）进行插管，并将静脉流出物引入储液器。右心室（RV）和左心室（LV）用荷包特灵法固定了大口径套管。在血液不能采取行动umula特在他们，一个. d收集在箱底部的流出物并返回到蓄水池。血液从储血器通过颈静脉返回到支持犬。通过刺伤将乳胶气球放入左心室。冠状窦管路中的针阀可升高冠状窦压力（PCS）。通过主动脉灌注插管中的侧臂记录平均冠状动脉压（Pa），通过与冠状窦口相同的流体静力学水平记录冠状窦导管中的侧臂记录平均冠状窦压，通过连接球囊与盒外的线记录左心室心室内压（Prv）。除非特别提及，否则通过仅使用小体积的球囊来保持较低的峰值脑室内压（小于25 mm Hg）。收缩力的变化表示为心室压力脉冲的dp/dt的变化百分比。在每个实验结束时，转动并称量左减ri的重量。切除心室，包括心尖、游离壁和室间隔乳头肌。流量表示为毫升/分钟/100 g LV。通过将橡胶尖端夹在套管上，使几毫升血液积聚在心室和管道中，并通过导管中的侧臂撤回。通过管路布置测量CS流量，以允许CS流出物在重力作用下流过流量计。通过在烧杯中定时收集流出液来测量RV和LV（thebesian）流量。血PO：！和pH值，并通过在Severinghaus列线图上插值来确定饱和度百分比。根据血红蛋白（1.36 X Hb）确定携氧能力;将耗氧量计算为AV差乘以三次流出中每一次的流量的乘积之和。在三个心脏中，将由此获得的结果与使用血氧计（IL 182型）测定氧含量获得的结果进行比较，结果无显著差异。在稳态下完成所有的“j”，。测量-
MATERIALS AND METHODSAn isolated, metabolically supported dog heart preparation (Fig. 1) was used. The methods of extracting the heart and suspending it by the trachea in a Lucite box for perfusion have been described previously (2 7). Heparinized blood from the carotid artery of an anesthetized(pentobarbitol 30 mg/kg iv) support dog is pumped into the aorta of the isolated heart where the aortic valves assure the blood is directed to the coronary system. The coronary sinus (CS) is cannulated and the venous effluent is brought to a reservoir. The right (RV) and left ventricles (LV) had largebore cannulas secured in them with purse-s tring sutures so th. at blood could not act umula te in them, an. d the effluent collected in the bottom of the box and returned to the reservoir. Blood from the reservoir returns to the support dog via the jugular vein. A latex balloon is placed in the left ventricle through a stab wound. A needle valve in the CS line allows coronary sinus pressure (PCS) to be raised. Mean coronary arte rial pressure (Pa) is recorded from a sidearm in the aortic perfusion cannula, mean coronary sinus pressure, from a sidearm in the coronary sinus catheter at the same hydrostatic level as the mouth of the coronary sinus, and left ventricular intraventricular pressure (Prv), through a line connecting the ballon to the outside of the box. Except when specifically mentioned, the peak intraventricular pressure was kept low (less than 25 mm Hg) by using only small volumes in the ballon. Changes in contractility were expressed as the percent change in the dp/dt of the ventricular pressure pulse. At the end of each experimen turn and t the left minus ri weighed. ventricle, including apex, free wall, and sepght ventricular papi llary muscles, was excised Flow was expressed as milliliters per min per 100 g LV.Oxygen consumption was measured with samples taken from the arterial side of the line, from the sidearm of the CS catheter, and from the ventricular effluents by clamping a rubber tip to the cannulas, allowing a few milliliters ol blood to accumulate in the ventricles and tubing, and withdrawing via the sidearm in the catheter. CS flow was measured by an arrangement of tubing such as to allow CS outflow to flow, gravitationally, through the flowmeter. RV and LV (thebesian) flows were measured by timed collection of the outflow in beakers. Blood PO:! and pH were measured and percent saturation was determined by interpolating on a Severinghaus nomogram. Oxygen carrying capacity was determined from the hemoglobin(1.36 X Hb); oxygen consumption was calculated as the sum of the products of the AV difference times the flow for each of the three outflows. In three hearts results thus obtained were compared with results obtained by using an oximeter (IL model 182) for determining oxygen content and were not significantly different. All urements of ‘j,, were done in the steady state. meas-