MEASUREMENT OF SHORT-CIRCUIT CURRENT + ION TRANSPORT ACROSS ILEUM
MEASUREMENT OF SHORT-CIRCUIT CURRENT + ION TRANSPORT ACROSS ILEUM
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DOI：
10.1152/ajplegacy.1964.206.3.658
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发表时间：
1964-01-01
影响因子：
--
通讯作者：
TOOLE, SR
中科院分区：
文献类型：
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作者：
CLARKSON, TW;TOOLE, SR
本文描述了一种装置，它能够控制大鼠离体回肠两侧的电势梯度和化学势梯度，同时还能测量离子和水的转运。从电极释放的银离子会影响离子转运。在没有络合剂的情况下，短路电流（SCC）在60分钟的测量期间下降，且等于净钠转运量。当沐浴液中有10⁻³摩尔/升还原型谷胱甘肽时，短路电流恒定，且等于钠转运量的94%。当有10⁻⁴摩尔/升半胱氨酸时，短路电流大幅增加。钠转运量也增加了，等于短路电流的75%，而氯离子分泌量等于短路电流的20%。文中提出了一种银离子刺激钠转运的机制。对开路和短路组织之间水盐转运的比较表明，当氯离子从黏膜侧向浆膜侧溶液直接通过组织转运（开路）时，比通过银/氯化银电极系统（短路实验）更能有效地产生水的移动。
An apparatus is described which allowed control over both the electrical and chemical potential gradients across the isolated ileum of the rat and at the same time permitted the measurement of the transport of ions and water. Silver ions, released from the electrodes, affected ion transport. In the absence of complexing agents, the short-circuit current (SCC) fell during the 60-min measurement period and was equal to the net sodium transport With 10-3 [image] reduced glutathione in the bathing solution, the SCC was constant and equal to 94% of the sodium transport With 10-4 [image]. cysteine, the SCC was greatly increased. Sodium transport, also increased, was equal to 75% and chloride secretion to 20% of the SCC. A mechanism by which silver ions stimulate sodium transport, is proposed. Comparison of water and salt transport between open- and short-circuited tissues indicated that chloride ions were more effective in producing water movement when transported from mucosal to serosal solutions directly through the tissue (open circuit) rather than via the Ag/AgCl electrode system (short-circuit experiments).