Method for the noninvasive measurement of intraocular pressure in mice
Method for the noninvasive measurement of intraocular pressure in mice
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DOI：
10.1167/iovs.02-0553
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发表时间：
2003-03-01
影响因子：
4.4
通讯作者：
Mittag, T
中科院分区：
文献类型：
--
作者：
Danias, J;Kontiola, AI;Mittag, T
目的.目的：评价反跳眼压计测量小鼠眼压的可行性。一个感应冲击（I/I）眼压计，其操作上的反弹的原则，按比例缩小，并适应于确定小鼠眼睛的IOP。使用该概念的IOP测量是基于用探针接触眼睛并检测探针与眼睛碰撞并弹回时的运动。探头的运动参数根据眼压而变化，并用于计算IOP。构建了用于测量小鼠眼睛中的IOP的原型仪器，并且通过将测量结果与在离体插管小鼠眼睛中测定的测压（真实）IOP进行比较来测试其准确且可靠地测量IOP的能力。I/I眼压计也用于在麻醉的成年C57 BL/6小鼠中测量体内IOP。在插管小鼠眼中，在3.7至44.1 mm Hg的范围内，发现真实IOP与I/I测量值（R-2 = 0.95）之间存在很强的相关性。在单个眼睛中重复测定显示，测量的IOP与真实IOP之间的关系具有较低程度的变异性。在麻醉小鼠中，当仅用氯胺酮麻醉动物时，通过回弹眼压测定法测定的平均IOP +/- SD为9.8 +/- 3.9 mm Hg，当使用氯胺酮、乙酰丙嗪和甲苯噻嗪的混合物时为7.6 +/- 1.9 mm Hg。对于两种麻醉方案，同侧眼分别相差0.9 ± 2.5和0.1 ± 2.7 mm Hg。I/I眼压计可用于小鼠眼中的非侵入性体内IOP测量。一个易于使用的，可靠的眼压计的可用性在小鼠中的IOP测量将允许更广泛地使用小鼠作为青光眼模型。
PURPOSE. To evaluate the applicability of rebound tonometry for measurement of IOP in the mouse eye.METHODS. An induction-impact (I/I) tonometer, which operates on the rebound principle, was scaled down and adapted to determine IOP of the mouse eye. IOP measurement using this concept is based on contacting the eye with a probe and detecting the motion as the probe collides with the eye and bounces back. The motion parameters of the probe vary according to eye pressure and are used to calculate IOP. A prototype instrument was constructed for measurement of IOP in mouse eyes, and its ability to accurately and reliably measure IOP was tested by comparing the measurements against the manometric (true) IOP determined in cannulated mouse eyes ex vivo. The I/I tonometer was also used to measure IOP in vivo in anesthetized adult C57BL/6 mice.RESULTS. A strong correlation between the true IOP and the I/I measurements (R-2 = 0.95) was found for IOPs in the range of 3.7 to 44.1 mm Hg in cannulated mouse eyes. Repeat determinations in individual eyes showed a low degree of variability in the relationship of the measured IOP with the true IOP. In anesthetized mice, mean IOP +/- SD as determined by rebound tonometry was 9.8 +/- 3.9 mm Hg when the animals were anesthetized with ketamine alone and 7.6 +/- 1.9 mm Hg when a mixture of ketamine, acepromazine, and xylazine was used. Contralateral eyes differed by 0.9 +/- 2.5 and 0.1 +/- 2.7 mm Hg, respectively, for the two anesthetic regimens.CONCLUSIONS. The I/I tonometer can be used for noninvasive, in vivo IOP measurement in mouse eyes. The availability of an easy-to-use, reliable tonometer for IOP measurements in mice will allow more extensive use of the mouse as a model for glaucoma.