Roles of CCL2 and CCL3 in intraocular inflammation during Bacillus endophthalmitis.
Roles of CCL2 and CCL3 in intraocular inflammation during Bacillus endophthalmitis.
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DOI：
10.1016/j.exer.2022.109213
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发表时间：
2022-11
影响因子：
3.4
通讯作者：
Callegan, Michelle C.
中科院分区：
文献类型：
--
作者：
Mursalin, Md Huzzatul;Astley, Roger;Coburn, Phillip S.;Miller, Frederick C.;Callegan, Michelle C.
蜡样芽孢杆菌（B. cereus）眼内炎是一种威胁视力的细菌感染。不受控制的炎症反应是这种疾病的标志，会对视网膜造成不可逆转的损害。我们最近报道了 C-X-C 趋化因子作为一种重要的调节剂，影响了这种疾病的发病机制。在这里，我们研究了两种高度上调的 C-C 趋化因子 CCL2 和 CCL3 对眼内炎症这种疾病的影响。将蜡状芽孢杆菌注射到 C57BL/6J (WT)、CCL2−/− 和 CCL3−/− 小鼠的眼睛中以诱导眼内炎。检查受感染眼睛的细菌生长、视网膜功能和炎症。 CCL2−/− 和 CCL3−/− 小鼠的细菌生长相似，但与 C57BL/6J 眼相比，CCL2−/− 和 CCL3−/− 眼保留的视网膜功能更大。 CCL2−/− 小鼠受感染眼睛的视网膜结构比受感染 CCL3−/− 小鼠眼睛的保存时间更长。受感染的 CCL2−/− 和 CCL3−/− 眼睛的炎症比受感染的 C57BL/6J 眼睛的炎症要少。根据这些结果，我们评估了玻璃体内注射抗 CCL2 或抗 CCL3 联合或不联合抗生素加替沙星的疗效。与受感染的未经治疗的眼睛相比，用加替沙星抗 CCL2 或抗 CCL3 治疗的眼睛的炎症明显减少，视网膜功能保留得更好。这项研究表明，CCL2−/− 小鼠的蜡样芽胞杆菌眼内炎比 CCL3−/− 小鼠的临床结果更好。玻璃体内注射抗 CCL2 和抗 CCL3 与加替沙星可显着减轻炎症并保护视网膜功能。这些结果表明，CCL2 和 CCL3 是潜在的抗炎靶标，应与其他抗生素一起进行测试，以治疗芽孢杆菌和其他形式的眼内炎。
Bacillus cereus (B. cereus) endophthalmitis is a vision-threatening bacterial infection. Uncontrolled inflammatory responses are the hallmark of this disease which cause irreversible damage to the retina. We recently reported C-X-C chemokines as a vital modulators which impacted the pathogenesis of this disease. Here, we investigated the impact of two highly upregulated C-C chemokines, CCL2 and CCL3, on intraocular inflammation this disease. B. cereus was injected into the eyes of C57BL/6J (WT), CCL2−/−, and CCL3−/− mice to induce endophthalmitis. Infected eyes were examined for bacterial growth, retinal function, and inflammation. Bacterial growth in CCL2−/− and CCL3−/− mice were similar, but retained retinal function was greater in CCL2−/− and CCL3−/− eyes compared to that of C57BL/6J eyes. The retinal architecture of infected eyes of CCL2−/− mice were conserved for a longer period of time than in infected CCL3−/− eyes. Infected CCL2−/− and CCL3−/− eyes had less inflammation than did infected C57BL/6J eyes. Based on these results, we assessed the efficacies of intravitreal anti-CCL2 or anti-CCL3 with or without the antibiotic gatifloxacin. Compared to infected untreated eyes, there was significantly less inflammation and greater retention of retinal function in eyes treated with anti-CCL2 or anti-CCL3 with gatifloxacin. This study showed that B. cereus endophthalmitis in CCL2−/− mice had a better clinical outcome than in CCL3−/− mice. Intravitreal administration of anti-CCL2 and anti-CCL3 with gatifloxacin significantly reduced inflammation and provided protection of retinal function. These results suggest that CCL2 and CCL3 are prospective anti-inflammatory targets that should be tested along with other antibiotics for treating Bacillus and perhaps other forms of endophthalmitis.
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影响因子：
--
作者：
Haringman, Jasper J.;Gerlag, Danielle M.;Tak, Paul P.
通讯作者：
Tak, Paul P.
影响因子：
3.1
作者：
Callegan, MC;Booth, MC;Gilmore, MS
通讯作者：
Gilmore, MS
DOI：
10.1099/mic.0.001057
发表时间：
2021-05
期刊：
Microbiology (Reading, England)
影响因子：
--
作者：
Coburn PS;Miller FC;Enty MA;Land C;LaGrow AL;Mursalin MH;Callegan MC
通讯作者：
Callegan MC
影响因子：
3.1
作者：
Callegan, MC;Cochran, DC;Lereclus, D
通讯作者：
Lereclus, D
影响因子：
3.1
作者：
Callegan, MC;Kane, ST;Lereclus, D
通讯作者：
Lereclus, D