The breadth, but not the magnitude, of circulating memory B cell responses to P. falciparum increases with age/exposure in an area of low transmission.
The breadth, but not the magnitude, of circulating memory B cell responses to P. falciparum increases with age/exposure in an area of low transmission.
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DOI：
10.1371/journal.pone.0025582
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Walther M
中科院分区：
文献类型：
--
作者：
Nogaro SI;Hafalla JC;Walther B;Remarque EJ;Tetteh KK;Conway DJ;Riley EM;Walther M
恶性疟原虫引起的疟疾仍然是撒哈拉以南非洲的主要死因。对疟疾症状的免疫需要反复接触，这表明寄生虫的免疫原性很差，或者对疟疾的有效免疫反应的发展可能受到损害。我们进行了两个年龄分层的抗疟疾体液免疫反应的横断面调查，在冈比亚的一个村庄，恶性疟原虫疟疾传播是低和零星的。用ELISA法和培养的B细胞ELISpot法检测血清循环抗体和记忆B细胞（MBC）。具有疟疾特异性MBC和抗体的个体的比例以及每个个体识别的抗原的平均数量随着年龄的增长而增加，但这些反应的幅度没有增加。疟疾特异性抗体水平与MBC的患病率或中位数均不相关，表明这两种检测方法测量的是体液免疫应答的不同方面。在那些有疟疾暴露的免疫学证据（定义为通过ELISA或ELISPOT对至少一种疟疾抗原的阳性反应）的患者中，疟疾特异性MBC的中位数与白喉特异性MBC的中位数相似，表明疟疾抗原的循环记忆细胞库与其他抗原的大小相似。
Malaria caused by Plasmodium falciparum remains a major cause of death in sub-Saharan Africa. Immunity against symptoms of malaria requires repeated exposure, suggesting either that the parasite is poorly immunogenic or that the development of effective immune responses to malaria may be impaired. We carried out two age-stratified cross-sectional surveys of anti-malarial humoral immune responses in a Gambian village where P. falciparum malaria transmission is low and sporadic. Circulating antibodies and memory B cells (MBC) to four malarial antigens were measured using ELISA and cultured B cell ELISpot. The proportion of individuals with malaria-specific MBC and antibodies, and the average number of antigens recognised by each individual, increased with age but the magnitude of these responses did not. Malaria-specific antibody levels did not correlate with either the prevalence or median number of MBC, indicating that these two assays are measuring different aspects of the humoral immune response. Among those with immunological evidence of malaria exposure (defined as a positive response to at least one malarial antigen either by ELISA or ELISPOT), the median number of malaria-specific MBC was similar to median numbers of diphtheria-specific MBC, suggesting that the circulating memory cell pool for malaria antigens is of similar size to that for other antigens.