Photoinduced Electron Transfer Reaction in Mitochondria for Spatiotemporal Selective Photo-Oxidation of Lipids by Donor/Acceptor Linked Molecules
Photoinduced Electron Transfer Reaction in Mitochondria for Spatiotemporal Selective Photo-Oxidation of Lipids by Donor/Acceptor Linked Molecules
复制标题
线粒体中的光诱导电子转移反应用于供体/受体连接分子对脂质的时空选择性光氧化
DOI：
10.1039/c7nr04919f
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发表时间：
2017
期刊：
影响因子：
6.7
通讯作者：
and H. Imahori
中科院分区：
文献类型：
--
作者：
Y. Takano;E. Hanai;and H. Imahori
已经合成了供体-受体连接分子，并利用光诱导分子间电子转移反应在线粒体中诱导快速和位点选择性的脂质氧化。为此，设计并合成了两个水溶性给受体分子（1和2）。2的制备是为了调节其对线粒体细胞膜的亲和力。激光共聚焦显微镜实验显示，1和2在线粒体中具有较高的定位能力。通过光诱导电子转移，2表现出显著的脂质氧化能力，主要是心磷脂。在HeLa细胞中，2在光照下触发线粒体脂质氧化，随后在几秒钟内导致细胞凋亡。这些结果表明，目前的分子体系非常有希望利用光在细胞内以精确的时空方式利用光诱导分子间电子转移反应。
Donor–acceptor-linked molecules have been synthesized and utilized to induce the rapid and site-selective lipid-oxidation in mitochondria by utilizing a photoinduced intermolecular electron transfer reaction. Two water-soluble donor–acceptor molecules (1 and 2) were designed and synthesized for this purpose. 2 was prepared to modulate its affinity to cell membrane in mitochondria. Confocal laser microscopy experiments revealed that 1 and 2 possess high localization abilities in mitochondria. By the photoinduced electron transfer, 2 exhibited the remarkable oxidation ability of lipids, mainly cardiolipin. In HeLa cells, 2 triggered mitochondrial lipid oxidation, which was followed by apoptotic cell death, under illumination within a few seconds. These results show that the present molecular system is highly promising to utilize the photoinduced intermolecular electron transfer reaction in a precise spatiotemporal manner in a cell by using light.