Cloning, purification and preliminary crystallographic analysis of cobalamin methyltransferases from Rhodobacter capsulatus.
Cloning, purification and preliminary crystallographic analysis of cobalamin methyltransferases from Rhodobacter capsulatus.
复制标题
荚膜红杆菌钴胺素甲基转移酶的克隆、纯化和初步晶体学分析。
DOI：
10.1107/s1744309110042910
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Seyedarabi A
中科院分区：
文献类型：
--
作者：
Seyedarabi A
在生产钴胺素（维生素B12）所需的30个生物合成步骤中，有8个涉及将S-腺苷甲硫氨酸衍生的甲基添加到四吡咯框架中。这八个甲基加成是由六个典型的甲基转移酶域和一个非典型的甲基转移酶域催化的。来自荚膜红细菌（Rhodobacter capsulatus）的四种甲基转移酶CobJ、CobM、CobF和CobL以及CobL的C-末端非典型结构域（CobL-C）的重组形式已经结晶，一些以多于一种晶体形式结晶。大多数晶体的衍射分辨率超过2.5 μ m，并且都适合于结构测定。 晶体的CobM和CobJ，这是参与环收缩，和CobL，这是参与两个甲基化和脱羧，首次报道。
Of the 30 biosynthetic steps necessary for the production of cobalamin (vitamin B12), eight involve the addition of S-adenosylmethionine-derived methyl groups to the tetrapyrrole framework. These eight methyl additions are catalysed by six canonical methyltransferase domains and one noncanonical methyltransferase domain. Recombinant forms of four methyltransferases from Rhodobacter capsulatus, CobJ, CobM, CobF and CobL, and of the C-terminal noncanonical domain of CobL (CobL-C) have been crystallized, some in more than one crystal form. Most of the crystals diffracted to beyond 2.5 Å resolution and all are suitable for structure determination. Crystals of CobM and CobJ, which are involved in ring contraction, and of CobL, which is involved in two methylations and decarboxylation, are reported for the first time.
影响因子：
5.6
作者：
J. Vévodová;R. Graham;E. Raux;H. Schubert;D. Roper;A. Brindley;A. Ian Scott;C. Roessner;N. Stamford;M. Elizabeth Stroupe;E. Getzoff;M. Warren;K. Wilson
通讯作者：
J. Vévodová;R. Graham;E. Raux;H. Schubert;D. Roper;A. Brindley;A. Ian Scott;C. Roessner;N. Stamford;M. Elizabeth Stroupe;E. Getzoff;M. Warren;K. Wilson