QTL analysis of net-like cracking in soybean seed coats
QTL analysis of net-like cracking in soybean seed coats
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DOI：
10.1270/jsbbs.60.28
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发表时间：
2010-03
期刊：
影响因子：
2.4
通讯作者：
M. Oyoo;S. Githiri;E. Benitez;R. Takahashi
中科院分区：
文献类型：
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作者：
M. Oyoo;S. Githiri;E. Benitez;R. Takahashi
大豆种皮裂纹有两种类型，受遗传控制：不规则裂纹的I型和网状裂纹的II型。这项研究的目的是使用DNA标记来确定II型开裂的遗传基础。本研究使用了具有网状裂纹的黑色种皮的Uzuramame与具有黑色种皮的Clark突变系杂交的F2群体和F3系。通过开裂指数（无开裂，0至严重开裂，4）对每个种子中的开裂程度进行评分，并对每株植物进行平均（平均开裂指数，ACI）。1个形态学标记和189个SSR标记进行了分析，并分配到31个连锁群，跨度为2463 cM。在分子连锁群C1上检测到2个ACI QTL（ncr1和ncr2），它们之间的距离约为62 cM。ncr1和ncr2的LOD值分别为5.90和8.61，分别占表型变异的16.0%和32.4%。来自Uzuramame的QTL等位基因赋予增加的ACI。在F2群体中鉴定的QTL的效应在F3系中得到证实。QTL之间没有上位性互作。
There are two types of seed coat cracking that are controlled genetically in soybean: Type I with irregular cracks and Type II with net-like cracks. This study was conducted to determine the genetic basis of Type II cracking using DNA markers. A F2 population and F3 lines derived from a cross between Uzuramame with black seed coats having net-like cracking, and a Clark mutant line with black seed coats were used in this study. Degree of cracking in each seed was scored by cracking index (no cracking, 0 to severe cracking, 4) and was averaged per plant (average cracking index, ACI). One morphological and 189 SSR markers were analyzed and assigned to 31 linkage groups spanning 2463 cM. Two QTLs for ACI (ncr1 and ncr2) were identified in molecular linkage group C1 with a distance of about 62 cM. The ncr1 and ncr2 had LOD scores of 5.90 and 8.61, accounting for 16.0 and 32.4% of phenotypic variation, respectively. The alleles from Uzuramame at the QTLs conferred increased ACI. The effects of QTLs identified in the F2 population were confirmed in the F3 lines. No epistatic interaction was observed between the QTLs.