Proliferation and phenotypic changes of stromal cells in response to varying estrogen/androgen levels in castrated rats.
Proliferation and phenotypic changes of stromal cells in response to varying estrogen/androgen levels in castrated rats.
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DOI：
10.1038/aja.2009.28
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发表时间：
2009-06
影响因子：
2.9
通讯作者：
Ying Zhou;Xiangqian Xiao;Linfeng Chen;Rui Yang;Jian-dang Shi;Xiao-Ling Du;H. Klocker;I. Park;Chung Lee;Ju Zhang
中科院分区：
文献类型：
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作者：
Ying Zhou;Xiangqian Xiao;Linfeng Chen;Rui Yang;Jian-dang Shi;Xiao-Ling Du;H. Klocker;I. Park;Chung Lee;Ju Zhang
已知人类良性前列腺增生可能是由雌激素/雄激素（E/T）失衡引起的。本文研究了去势大鼠前列腺对不同E/T比值的反应。雄性Wistar去势大鼠随机注射不同比例的E/T，连续4周。前列腺指数、苏木精-伊红染色及α-平滑肌肌动蛋白（SMA）免疫组化定量分析显示，E/T（1：100）组前列腺增生明显。在该组中，波形蛋白、非肌肌球蛋白重链（NMMHC）和增殖细胞核抗原（PCNA）阳性细胞在间质和上皮中增加。平滑肌肌球蛋白重链（SMMHC）和NMMHC的mRNA表达水平增加。因此，1：100比例的E/T可诱导去势大鼠前列腺基质增生反应。观察到的主要变化是平滑肌细胞增加，而在大鼠前列腺中也观察到一些上皮变化。
It is known that human benign prostatic hyperplasia might arise from an estrogen/androgen (E/T) imbalance. We studied the response of castrated rat prostate to different ratios of circulating E/T. The castrated male Wistar rats were randomly injected with E/T at different ratios for 4 weeks. The prostates of E/T (1:100) group showed a distinct prostatic hyperplasia response by prostatic index, hematoxylin and eosin staining, and quantitative immunohistochemical analysis of α-smooth muscle actin (SMA). In this group, cells positive for Vimentin, non-muscle myosin heavy chain (NMMHC) and proliferating cell nuclear antigen (PCNA) increased in the stroma and epithelium. Furthermore, the mRNA levels of smooth muscle myosin heavy chain (SMMHC) and NMMHC increased. So E/T at a ratio of 1:100 can induce a stromal hyperplastic response in the prostate of castrated rats. The main change observed was an increase of smooth muscle cells, whereas some epithelial changes were also seen in the rat prostates.