Calcium-dependent activation of glycogen phosphorylase in rat pheochromocytoma PC12 cells by nerve growth factor.
Calcium-dependent activation of glycogen phosphorylase in rat pheochromocytoma PC12 cells by nerve growth factor.
复制标题
神经生长因子对大鼠嗜铬细胞瘤 PC12 细胞中糖原磷酸化酶的钙依赖性激活。
DOI：
10.1016/s0006-291x(86)80583-6
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Kauffman,FC
中科院分区：
文献类型：
--
作者：
Davis,LH;Kauffman,FC
糖原磷酸化酶在PC 12细胞中以两种形式存在，类似于在脑和肌肉中发现的糖原磷酸化酶。活性磷酸化形式的酶，磷酸化酶-a，占这些细胞中总糖原磷酸化酶的约20-30%。用100 ng 7S神经生长因子/ml孵育PC 12细胞在数分钟内增加磷酸化酶-a。与神经生长因子相反，胰岛素（6 ng/ml）和表皮生长因子（6 ng/ml）降低磷酸化酶-a。磷酸化酶-a的神经生长因子的激活并不伴随着环AMP的增加，然而，细胞外Ca 2+的去除或与钙通道阻滞剂的细胞孵育抑制神经生长因子的糖原磷酸化酶的激活。
Glycogen phosphorylase in PC12 cells exists in two forms analogous to those found in brain and muscle. The active phosphorylated form of the enzyme, phosphorylase-a, represents about 20–30% of total glycogen phosphorylase in these cells. Incubation of PC12 cells with 100 ng 7S nerve growth factor/ml increased phosphorylase-a within minutes. In contrast to nerve growth factor, insulin (6 ng/ml) and epidermal growth factor (6 ng/ml) decreased phosphorylase-a. Activation of phosphorylase-a by nerve growth factor was not accompanied by increases in cyclic AMP; however, removal of extracellular Ca2+or incubation of cells with calcium channel blockers inhibited activation of glycogen phosphorylase by nerve growth factor.