The Genome Segments of a Group D Rotavirus Possess Group A-Like Conserved Termini but Encode Group-Specific Proteins
The Genome Segments of a Group D Rotavirus Possess Group A-Like Conserved Termini but Encode Group-Specific Proteins
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DOI：
10.1128/jvi.00332-10
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发表时间：
2010-10-01
影响因子：
5.4
通讯作者：
Johne, Reimar
中科院分区：
文献类型：
--
作者：
Trojnar, Eva;Otto, Peter;Johne, Reimar
轮状病毒是人类和动物病毒性急性胃肠炎的主要原因。它们根据VP6的基因组成和抗原性进行分组。A组、B组和C组轮状病毒存在于人类和动物中，而D组轮状病毒仅在鸟类中检测到。尽管它们在鸡中广泛分布，但到目前为止还没有核苷酸序列数据。在这里，第一个完整的基因组序列的D组轮状病毒（株05V0049），这是扩增使用序列独立的扩增策略和简并引物。鉴定了编码轮状病毒蛋白VP1至VP4、VP6、VP7和NSP1至NSP5的同源物的开放阅读框。D组轮状病毒与A组、B组和C组轮状病毒的氨基酸序列同源性分别为12.3%和51.7%、11.0%和23.1%、9.5%和46.9%。D组轮状病毒的区段10具有额外的开放阅读框。一般来说，系统发育分析表明A、C和D组轮状病毒的共同进化，与B组的进化分开。然而，C组的NSP4序列与所有其它组的有说服力的序列相比仅具有非常低的同一性。禽类A组NSP1序列与D组NSP1序列的关系比哺乳动物A组轮状病毒的关系更密切。最有趣的是，D组和A组轮状病毒的11个基因组片段末端的核苷酸序列是相同的。进一步的研究应该澄清这些保守的结构是否允许A组和D组轮状病毒之间的基因组片段的交换。
Rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. They are grouped according to gene composition and antigenicity of VP6. Whereas group A, B, and C rotaviruses are found in humans and animals, group D rotaviruses have been exclusively detected in birds. Despite their broad distribution among chickens, no nucleotide sequence data exist so far. Here, the first complete genome sequence of a group D rotavirus (strain 05V0049) is presented, which was amplified using sequence-independent amplification strategies and degenerate primers. Open reading frames encoding homologues of rotavirus proteins VP1 to VP4, VP6, VP7, and NSP1 to NSP5 were identified. Amino acid sequence identities between the group D rotavirus and the group A, B, and C rotaviruses varied between 12.3% and 51.7%, 11.0% and 23.1%, and 9.5% and 46.9%, respectively. Segment 10 of the group D rotavirus has an additional open reading frame. Generally, phylogenetic analysis indicated a common evolution of group A, C, and D rotaviruses, separate from that of group B. However, the NSP4 sequence of group C has only very low identities in comparison with cogent sequences of all other groups. The avian group A NSP1 sequences are more closely related to those of group D than those of mammalian group A rotaviruses. Most interestingly, the nucleotide sequences at the termini of the 11 genome segments are identical between group D and group A rotaviruses. Further investigations should clarify whether these conserved structures allow an exchange of genome segments between group A and group D rotaviruses.