Estimation of the novel antipyretic, anti-inflammatory, antinociceptive and antihyperlipidemic effects of silymarin in Albino rats and mice
Estimation of the novel antipyretic, anti-inflammatory, antinociceptive and antihyperlipidemic effects of silymarin in Albino rats and mice
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DOI：
10.1016/j.apjtb.2015.05.009
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发表时间：
2015-08-01
影响因子：
1.7
通讯作者：
Arbid, Mahmoud Soliman
中科院分区：
文献类型：
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作者：
Amin, Mohamed Mahmoud;Arbid, Mahmoud Soliman
目的：评价水飞蓟素对Albino大鼠和小鼠的解热、抗炎、镇痛、降血脂等其他药理作用。方法：给大鼠肌肉注射致热剂量的啤酒酵母进行水飞蓟素的解热试验。另一组大鼠在右后爪种植区注射0.1 mL 1%角叉菜胶生理盐水，进行水飞蓟素的抗炎试验。另一组小鼠采用热板法测定水飞蓟素的镇痛作用。采用高脂饮食诱导高脂血症2个月，以评估水飞蓟素的降血脂活性。结果：与对照组相比，水飞蓟素在两个剂量（50和100 mg/kg）下均显示出显着的解热作用。此外，与对照组阳性组相比，水飞蓟素阐明了两种剂量的显着抗炎作用，这反映在给药后4小时内每小时间隔一次的大鼠爪子水肿的减少。同样，在热板法中，两种剂量的水飞蓟素在给药后 30 和 60 分钟均显示出显着的镇痛作用。此外，水飞蓟素给药2周后，除了显着降低总胆固醇、甘油三酯、低密度脂蛋白和显着升高的高密度脂蛋白外，在角叉菜胶诱导的大鼠足肿胀中，给药2小时后，显着降低前列腺素E2、肿瘤坏死因子α和白细胞介素1β的血清水平。 结果为水飞蓟素促进解热、抗炎、镇痛和降血脂作用的可能药理机制提供了新的视角。
Objective: To evaluate the other pharmacological actions of silymarin in Albino rats and mice such as antipyretic, anti-inflammatory, antinociceptive and antihyperlipidemic effects.Methods: Rats were injected intramuscularly with pyrogenic dose of brewer's yeast for the antipyretic test of silymarin. Another group of rats injected with 0.1 mL of 1% carrageenan solution in saline at the subplanter area of the right hind paw for the antiinflammatory test of silymarin. Another group of mice tested by hot plate method for determination of antinociceptive effect of silymarin. Hyperlipidemia was induced using high fat diet for 2 months to estimate the antihyperlipidemic activity of silymarin.Results: Silymarin showed a significant antipyretic effect of both doses (50 and 100 mg/kg) compared with control untreated group. Moreover, silymarin elucidated a significant anti-inflammatory effect of both doses reflected on the decrease of the rat paw edema every hour interval for 4 h after administration in comparison with control positive group. By the same taken, both doses of silymarine revealed a significant antinociceptive action in hot plate method at 30 and 60 min post administration. Besides, it lowered significantly the serum levels of prostaglandin E2, tumor necrosis factor alpha and interleukin 1 beta after 2 h of silymarin administration in carrageenan induced rat paw edema besides the significant decrease of total cholesterol, triglycerides, low density lipoprotein and significantly elevated high density lipoprotein after 2 weeks of silymarin administration.Conclusions: These outcomes delivered a new vision into the possible pharmacological mechanisms by which silymarin advances antipyretic, anti-inflammatory, antinociceptive and antihyperlipidemic effects.