Direct Analysis of Phycobilisomal Antenna Proteins and Metabolites in Small Cyanobacterial Populations by Laser Ablation Electrospray Ionization Mass Spectrometry
Direct Analysis of Phycobilisomal Antenna Proteins and Metabolites in Small Cyanobacterial Populations by Laser Ablation Electrospray Ionization Mass Spectrometry
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DOI：
10.1021/ac202831w
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发表时间：
2012-01-03
影响因子：
7.4
通讯作者：
Vertes, Akos
中科院分区：
文献类型：
--
作者：
Parsiegla, Goetz;Shrestha, Bindesh;Vertes, Akos
由于蓝藻在能源、环境和天然产物研究中的重要性，以及其巨大的遗传多样性，蓝藻的快速原位分析越来越受到人们的关注。需要了解能量收集天线蛋白复合物的代谢特征和组成，以了解环境因素如何影响这些微生物的功能。在这里，我们表明，激光烧蚀电喷雾电离（LAESI）质谱能够直接分析藻胆体天线蛋白和报告的许多代谢产物从完整的蓝藻。通过LAESI质谱法分析营养性鱼腥藻属PCC 7120蓝藻细胞的小群体（n < 616 +/-76）。光谱揭示的比率藻蓝蛋白（C-PC）和别藻蓝蛋白（APC）的天线复合体，亚基组成的藻胆蛋白，和超过30个代谢产物和脂质的暂定身份。通过精确的质量测量、同位素分布模式和文献检索，初步确定了金属同位素。快速同时分析丰富的蛋白质和不同的代谢产物，使评估的环境响应和代谢适应蓝藻和其他微生物。
Due to their significance in energy and environmental and natural product research, as well as their large genetic diversity, rapid in situ analysis of cyanobacteria is of increasing interest. Metabolic profiles and the composition of energy harvesting antenna protein complexes are needed to understand how environmental factors affect the functioning of these microorganisms. Here, we show that laser ablation electrospray ionization (LAESI) mass spectrometry enables the direct analysis of phycobilisomal antenna proteins and report on numerous metabolites from intact cyanobacteria. Small populations (n < 616 +/- 76) of vegetative Anabaena sp. PCC7120 cyanobacterial cells are analyzed by LAESI mass spectrometry. The spectra reveal the ratio of phycocyanin (C-PC) and allophycocyanin (APC) in the antenna complex, the subunit composition of the phycobiliproteins, and the tentative identity of over 30 metabolites and lipids. Metabolites are tentatively identified by accurate mass measurements, isotope distribution patterns, and literature searches. The rapid simultaneous analysis of abundant proteins and diverse metabolites enables the evaluation of the environmental response and metabolic adaptation of cyanobacteria and other microorganisms.