Sex-dependent and independent effects of long-term voluntary wheel running on Bdnf mRNA and protein expression.
Sex-dependent and independent effects of long-term voluntary wheel running on Bdnf mRNA and protein expression.
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DOI：
10.1016/j.physbeh.2015.12.026
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发表时间：
2016-03-15
影响因子：
2.9
通讯作者：
Roth SM
中科院分区：
文献类型：
--
作者：
Venezia AC;Guth LM;Sapp RM;Spangenburg EE;Roth SM
身体活动对大脑健康的有益影响（突触发生，神经发生，增强突触可塑性，改善学习和记忆）似乎是通过神经营养因子，转录因子和突触后受体的区域特异性表达的变化来介导的，尽管对长期自愿轮运行的性别差异的调查有限。研究5个月自愿轮跑对雄性和雌性成年小鼠海马mRNA和蛋白表达的影响，这些因子对运动诱导的结构和功能可塑性至关重要。在8周龄时，将雄性和雌性C57 BL/6小鼠单独圈养，其中（PA; n=20; 10只雄性）或不（SED; n=20; 10只雄性）使用计算机监控的自主跑步轮。在28周时，处死所有小鼠并移除盲肠。从海马中分离总RNA，用定量RT-PCR评估总BDNF、BDNF转录本IV、tPA、Pgc-1a、GluR 1、NR 2A和NR 2B的表达，用ELISA评估总BDNF蛋白和成熟BDNF蛋白。我们发现，PA男性（p=0.03）和女性（p=0.03）相比，SED动物Bdnf IV mRNA的表达显着较高。总BDNF mRNA的表达显着更大的PA男性相比，SED男性（p=0.01），但在女性中没有差异。同样，我们观察到显着较高的成熟BDNF蛋白在PA男性相比，SED男性（p=0.04），但不是在女性。这些研究结果表明，长期自愿轮运行对转录和翻译后调节的BDNF的影响可能是性别依赖性的，虽然活动依赖的BDNF IV转录是敏感的运动独立的性别。
The beneficial effects of physical activity on brain health (synaptogenesis, neurogenesis, enhanced synaptic plasticity, improved learning and memory) appear to be mediated through changes in region-specific expression of neurotrophins, transcription factors, and postsynaptic receptors, though investigations of sex differences in response to long-term voluntary wheel running are limited. To examine the effect of five months of voluntary wheel running on hippocampal mRNA and protein expression of factors critical for exercise-induced structural and functional plasticity in male and female adult mice. At 8 weeks of age, male and female C57BL/6 mice were individually housed with (PA; n=20; 10 male) or without (SED; n=20; 10 male) access to a computer monitored voluntary running wheel. At 28 weeks, all mice were sacrificed and hippocampi removed. Total RNA was isolated from the hippocampus and expression of total Bdnf, Bdnf transcript IV, tPA, Pgc-1a, GluR1, NR2A, and NR2B were assessed with quantitative RT-PCR and total and mature Bdnf protein were assessed with ELISA. We found significantly higher Bdnf IV mRNA expression in PA males (p=0.03) and females (p=0.03) compared to SED animals. Total Bdnf mRNA expression was significantly greater in PA males compared to SED males (p=0.01), but there was no difference in females. Similarly, we observed significantly higher mature Bdnf protein in PA males compared to SED males (p=0.04), but not in females. These findings indicate that the impact of long-term voluntary wheel running on transcriptional and post-translational regulation of Bdnf may be sex-dependent, though the activity-dependent Bdnf IV transcript is sensitive to exercise independent of sex.