Serum Glycome Profiling: A Biomarker for Diagnosis of Ovarian Cancer
Serum Glycome Profiling: A Biomarker for Diagnosis of Ovarian Cancer
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DOI：
10.1021/pr400405x
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发表时间：
2013-09-01
影响因子：
4.4
通讯作者：
Blanchard, Veronique
中科院分区：
文献类型：
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作者：
Biskup, Karina;Braicu, Elena I.;Blanchard, Veronique
在癌症的发展过程中，观察到细胞糖基化的变化，表明糖组的变化发生在细胞外液和血清中，因此可以作为肿瘤生物标志物。就上皮性卵巢癌（EOC）而言，CA125等常见肿瘤标志物特异性较差；因此，需要更好的生物标志物。这项工作的目的是鉴定 EOC 患者中新的潜在聚糖生物标志物。从 63 名术前原发性 EOC 患者和 33 名年龄匹配的健康女性的血清糖蛋白中切下 N-聚糖，进行全甲基化，并使用 MALDI-TOF 质谱法进行分析。根据 SPSS 统计分析显示的 11 个 N-聚糖生物标志物（即 4 个高甘露糖和 7 个复合型岩藻糖基化 N-聚糖）的相对面积计算出名为 GLYCOV 的值。 GLYCOV 诊断原发性 EOC 的敏感性为 97%，特异性为 98.4%，而 CA-125 的敏感性为 97%，特异性为 88.9%。我们的研究是第一个将聚糖值与已建立的肿瘤标记物 CA125 进行比较并给出更好的结果的研究。因此，N-糖组有可能用作生物标志物。
During the development of cancer, changes in cellular glycosylation are observed, indicating that alterations of the glycome occur in extracellular fluids as well as in serum and could therefore serve as tumor biomarkers. In the case of epithelial ovarian cancer (EOC), common tumor markers such as CA125 are known to have poor specificity; therefore, better biomarkers are needed. The aim of this work was to identify new potential glycan biomarkers in EOC-patients. N-Glycans were cleaved from serum glycoproteins from 63 preoperative primary EOC-patients along with 33 age-matched healthy women, permethylated, and analyzed using MALDI-TOF mass spectrometry. A value named GLYCOV was calculated from the relative areas of the 11 N-glycan biomarkers revealed by SPSS statistical analyses, namely four high-mannose and seven complex-type fucosylated N-glycans. GLYCOV diagnosed primary EOC with a sensitivity of 97% and a specificity of 98.4% whereas CA-125 showed a sensitivity of 97% and a specificity of 88.9%. Our study is the first one to compare glycan values with the established tumor marker CA125 and to give better results. Therefore, the N-glycome could potentially be used as a biomarker.