Aeromonas aquatica sp nov., Aeromonas finlandiensis sp nov and Aeromonas lacus sp nov isolated from Finnish waters associated with cyanobacterial blooms

Aeromonas aquatica sp nov., Aeromonas finlandiensis sp nov and Aeromonas lacus sp nov isolated from Finnish waters associated with cyanobacterial blooms
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DOI:
10.1016/j.syapm.2015.02.005
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发表时间:
2015-05-01
影响因子:
3.4
通讯作者:
Figueras, M. J.
Figueras, M. J.
中科院分区:
生物学2区
文献类型:
--
作者:
Beaz-Hidalgo, R.;Latif-Eugenin, F.;Figueras, M. J.

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三组气单胞菌菌株分离自芬兰湖泊经历蓝藻水华不能被分配到任何已知的物种,本属的16 S rRNA和rpoD基因序列的基础上。对7个基因(gyrB、rpoD、recA、dnaJ、gyrA、dnaX和atpD; 4093 bp)的串联序列进行多位点系统发育分析(MLPA),结果表明3组菌株均未与任何已知的气单胞菌属(Aeromonas spp.)形成了三个独立的谱系。这通过使用15个基因(后7个和cpn 60、dnaK、gltA、mdh、radA、rpoB、tsf、zipA; 8751 bp)与它们的最近亲属进行分析来证实。此外,三个潜在新种的模式菌株与其近缘种的基因组间的ANI结果均为阴性。
Three groups of Aeromonas strains isolated from Finland lakes experiencing cyanobacterial blooms could not be assigned to any known species of this genus on the basis of 16S rRNA and rpoD gene sequences. The Multilocus Phylogenetic Analysis (MLPA) of the concatenated sequence of seven genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX and atpD; 4093 bp) showed that the three groups of strains did not cluster with any known Aeromonas spp. and formed three independent lineages. This was confirmed by performing the analysis with their closest relatives using 15 genes (the latter 7 and cpn60, dnaK, gltA, mdh, radA, rpoB, tsf, zipA; 8751 bp). Furthermore, ANI results between the genomes of the type strains of the three potential new species and those of their close relatives were all