Epithelial eotaxin-2 and eotaxin-3 expression: relation to asthma severity, luminal eosinophilia and age at onset.
Epithelial eotaxin-2 and eotaxin-3 expression: relation to asthma severity, luminal eosinophilia and age at onset.
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DOI：
10.1136/thoraxjnl-2012-201634
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发表时间：
2012-12
期刊：
影响因子：
10
通讯作者：
Wenzel SE
中科院分区：
文献类型：
--
作者：
Coleman JM;Naik C;Holguin F;Ray A;Ray P;Trudeau JB;Wenzel SE
Eosinophilic inflammation is implicated in asthma. Eotaxin 1–3 regulate eosinophil trafficking into the airways along with other chemotactic factors. However, the epithelial and bronchoalveolar lavage (BAL) cell expression of these chemokines in relation to asthma severity and eosinophilic phenotypes has not been addressed. To measure the expression of the three eotaxin isoforms in bronchoscopically obtained samples and compare them with clinically relevant parameters between normal subjects and patients with asthma. Normal subjects and patients with asthma of varying severity recruited through the Severe Asthma Research Program underwent clinical assessment and bronchoscopy with airway brushing and BAL. Eotaxin 1–3 mRNA/protein were measured in epithelial and BAL cells and compared with asthma severity, control and eosinophilic inflammation. Eotaxin-2 and eotaxin-3 mRNA and eotaxin-2 protein were increased in airway epithelial brushings from patients with asthma and were highest in cases of severe asthma (p values 0.0155, 0.0033 and 0.0006, respectively), with eotaxin-2 protein increased with age at onset. BAL cells normally expressed high levels of eotaxin-2 mRNA/protein but BAL fluid levels of eotaxin-2 were lowest in severe asthma. Epithelial eotaxin-2 and eotaxin-3 mRNA/protein was associated with sputum eosinophilia, lower forced expiratory volume in 1 s and more asthma exacerbations. Airway epithelial cell eotaxin-2 protein differed by asthma severity only in those with late onset disease, and tended to be highest in those with late onset eosinophilic asthma. Epithelial eotaxin-2 and 3 are increased in asthma and severe asthma. Their expression may contribute to luminal migration of eosinophils, especially in later onset disease, asthma control and severity.
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DOI：
10.1016/j.jaci.2011.02.042
发表时间：
2011-08
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Moore WC;Evans MD;Bleecker ER;Busse WW;Calhoun WJ;Castro M;Chung KF;Erzurum SC;Curran-Everett D;Dweik RA;Gaston B;Hew M;Israel E;Mayse ML;Pascual RM;Peters SP;Silveira L;Wenzel SE;Jarjour NN;National Heart, Lung, and Blood Institute's Severe Asthma Research Group
通讯作者：
National Heart, Lung, and Blood Institute's Severe Asthma Research Group
影响因子：
15.9
作者：
Lilly, CM;Nakamura, H;Luster, AD
通讯作者：
Luster, AD
影响因子：
24.3
作者：
Scheicher, M. E.;Teixeira, M. M.;Vianna, E. O.
通讯作者：
Vianna, E. O.
DOI：
10.1164/rccm.201103-0396oc
发表时间：
2011-11-15
影响因子：
24.7
作者：
Castro, Mario;Mathur, Sameer;Nair, Parameswaran
通讯作者：
Nair, Parameswaran
DOI：
10.1056/nejmoa0808991
发表时间：
2009-03-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
Haldar P;Brightling CE;Hargadon B;Gupta S;Monteiro W;Sousa A;Marshall RP;Bradding P;Green RH;Wardlaw AJ;Pavord ID
通讯作者：
Pavord ID