Intracytoplasmic delivery of anionic proteins
Intracytoplasmic delivery of anionic proteins
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DOI：
10.1016/j.ymthe.2004.03.007
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发表时间：
2004-06-01
影响因子：
12.4
通讯作者：
Behr, JP
中科院分区：
文献类型：
--
作者：
Dalkara, D;Zuber, G;Behr, JP
蛋白质传递正在成为基因传递的一种有趣的替代方案。我们利用转染方面的经验，开发了一种将阴离子蛋白（如抗体）高效递送到细胞质中的技术。至于DNA，当与阳离子脂质络合时，大量蛋白质通过无处不在表达的syndecans进入贴壁细胞。然而，蛋白质的表面积而不是电荷比决定了传递特性。抗-肌动蛋白和抗-微管蛋白IgG的递送导致纤维解聚。因此，细胞内递送抗体可视为干扰基因活性的另一种方法。
Protein delivery is emerging as an interesting alternative to gene delivery. We have used our experience with transfection to develop a technique for efficient delivery of anionic proteins such as antibodies into the cytoplasm of cells. As for DNA, when complexed with cationic lipids, large amounts of proteins are shown to enter adherent cells via ubiquitously expressed syndecans. However, protein surface area rather than electric charge ratio governs the delivery characteristics. Delivery of anti-beta-actin and anti-alpha-tubulin IgG's leads to fiber depolymerization. Intracellular delivery of an antibody could thus be regarded as another method for interfering with gene activity.