Isolation of adult mouse myogenic progenitors: Functional heterogeneity of cells within and engrafting skeletal muscle
Isolation of adult mouse myogenic progenitors: Functional heterogeneity of cells within and engrafting skeletal muscle
复制标题
DOI：
10.1016/j.cell.2004.10.021
复制
发表时间：
2004-11-12
期刊：
影响因子：
64.5
通讯作者：
Wagers, AJ
中科院分区：
文献类型：
--
作者：
Sherwood, RI;Christensen, JL;Wagers, AJ
成人骨骼肌再生被认为是通过与成熟肌纤维密切相关的肌原性卫星细胞的作用发生的；然而，这些前体细胞尚未被前瞻性地分离出来，最近的研究表明，可能存在其他肌肉祖细胞，包括骨髓细胞或造血细胞。为了阐明成人肌源性细胞的起源，我们使用表型、形态学和功能标准来鉴定和前瞻性地分离出一组肌纤维相关细胞，这些细胞能够在单细胞水平上高频地产生肌源性集落。重要的是，尽管来自骨髓和/或循环的肌肉移植细胞定位于与肌原性卫星细胞相同的解剖腔室，并表达一些（尽管不是全部）卫星细胞标记，但它们没有表现出内在的肌原性。总之，这些研究描述了功能性成人肌源性祖细胞的克隆分离，并证明这些细胞不是来自造血或其他骨髓或循环前体。
Skeletal muscle regeneration in adults is thought to occur through the action of myogenic satellite cells located in close association with mature musclefibers; however, these precursor cells have not been prospectively isolated, and recent studies have suggested that additional muscle progenitors, including cells of bone marrow or hematopoietic origin, may exist. To clarify the origin(s) of adult myogenic cells, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of myofiber-associated cells capable at the single cell level of generating myogenic colonies at high frequency. Importantly, although muscle-engrafted cells from marrow and/or circulation localized to the same anatomic compartment as myogenic satellite cells and expressed some though not all satellite cell markers, they displayed no intrinsic myogenicity. Together, these studies describe the clonal isolation of functional adult myogenic progenitors and demonstrate that these cells do not arise from hematopoietic or other bone marrow or circulating precursors.