Glutamate receptors and calcium entry mechanisms for domoic acid in hippocampal neurons.
Glutamate receptors and calcium entry mechanisms for domoic acid in hippocampal neurons.
复制标题
海马神经元中软骨藻酸的谷氨酸受体和钙进入机制。
DOI：
10.1097/00001756-199604260-00002
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发表时间：
1996
期刊：
影响因子：
1.7
通讯作者：
Ramsdell,JS
中科院分区：
文献类型：
--
作者：
Xi,D;Ramsdell,JS
DOMOIC酸（50 nM）升高了49%的从出生后第1天（PND 1）大鼠分离的海马锥体神经元中的胞质游离钙（[Ca 2 +] i）水平。这种作用可被6-氰基-7-硝基喹喔啉-2，3-二酮（CNQX;一种非N-甲基-D-天冬氨酸（NMDA）受体的拮抗剂）阻止，但不能被2-氨基-5-膦酰基戊酸（AP-5;一种NMDA受体的拮抗剂）阻止。软骨藻酸在5 [mu] M也升高[Ca 2 +] i水平在第二个群体（36%）的神经元，其中的效果仅部分抑制100 [mu] M CNQX。300 nM尼莫地平可阻止50 nM和5 [mu] M软骨藻酸引起的[Ca 2 +] i升高，表明软骨藻酸通过L型电压依赖性钙通道诱导Ca 2+进入。这些结果为新生儿海马锥体细胞原代培养物中至少两种软骨藻酸敏感性非NMDA受体亚型提供了证据，并表明电压依赖性钙通道是软骨藻酸作用的主要钙进入机制。
DOMOIC acid (50 nM) elevates cytosolic free calcium ([Ca2+] i) levels in 49% of the hippocampal pyramidal neurons isolated from postnatal day one (PND1) rats. This effect was prevented by 6-cyano-7-nitroquinoxaline-2, 3-dione (CNQX; an antagonist of non-N-methyl-D-aspartate (NMDA) receptors, but not 2-amino-5-phosphonovaleric acid (AP-5; an antagonist of NMDA receptors). Domoic acid given at 5 [mu] M also elevated [Ca2+] i levels in a second population (36%) of neurons in which the effect was only partially inhibited by 100 [mu] M CNQX. Nimodipine given at 300 nM prevented the elevation in [Ca2+] i caused by 50 nM and 5 [mu] M domoic acid, indicating that domoic acid induced Ca2+ entry through type L voltage dependent calcium channels. These results provide evidence for at least two domoic acid-sensitive non-NMDA receptor subtypes in primary cultures of neonatal hippocampal pyramidal cells and indicate that voltage-dependent calcium channels are a primary calcium entry mechanism for domoic acid action.