Blood Circulation and Tissue Biodistribution of Lipid-Quantum Dot (L-QD) Hybrid Vesicles Intravenously Administered in Mice
Blood Circulation and Tissue Biodistribution of Lipid-Quantum Dot (L-QD) Hybrid Vesicles Intravenously Administered in Mice
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DOI：
10.1021/bc900047n
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发表时间：
2009-09-01
影响因子：
4.7
通讯作者：
Kostarelos, Kostas
中科院分区：
文献类型：
--
作者：
Al-Jamal, Wafa T.;Al-Jamal, Khuloud T.;Kostarelos, Kostas
本文描述了最近开发的脂质体-量子点（L-QD）杂交囊泡在裸鼠全身给药后的药代动力学。将疏水性QD掺入到不同的双层组成中，并通过浊度和羧基荧光素释放测量来评估这种混合囊泡的血清稳定性。L-QD杂交血谱和器官生物分布也通过元素（镉）分析测定。静脉给药后，根据L-QD脂质双分子层特征，观察到不同的组织生物分布概况和组织亲和力。阳离子（DOTAP/DOPE/Chol）混合物可立即在血液中清除并迅速积聚，而在两性离子囊泡表面掺入PEG可显着延长其全身给药后的血液循环半衰期。总的来说，L-QD杂交囊泡系统被认为是一个可行的平台，通过杂交囊泡特性的简单调节，可以将QD输送到不同的组织。此外，L-QD通过在单个囊泡的不同腔室内结合药物分子和QD，为开发组合治疗和成像（治疗）模式提供了许多机会。
The present work describes the pharmacokinctics of recently developed liposome-quantum dot (L-QD) hybrid vesicles in nude mice following systemic administration. Hydrophobic QD were incorporated into different bilayer compositions, and the serum stability of such hybrid vesicles was evaluated using turbidity and carboxyfluorescein release measurements. L-QD hybrid blood profile and organ biodistribution were also determined by elemental (cadmium) analysis. Following intravenous administration, different tissue biodistribution profiles and tissue affinities were observed depending on the L-QD lipid bilayer characteristics. Immediate blood clearance was observed with cationic (DOTAP/DOPE/Chol) hybrid with rapid lung accumulation, while incorporation of PEG at the surface of zwitterionic vesicles dramatically prolonged their blood circulation half-life after systemic administration. Overall, the L-QD hybrid vesicle system is considered a viable platform that allows QD delivery to different tissues through facile modulation of the hybrid vesicle characteristics. In addition, L-QD offers many opportunities for the development of combinatory therapeutic and imaging (theranostic) modalities by incorporating both drug molecules and QD within the different compartments of a single vesicle.