Phenotypic and functional alterations of monocyte subsets with aging.
Phenotypic and functional alterations of monocyte subsets with aging.
复制标题
单核细胞亚群随衰老的表型和功能变化
DOI：
10.1186/s12979-022-00321-9
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发表时间：
2022-12-13
影响因子：
7.9
通讯作者：
Zeng, Hui
中科院分区：
文献类型：
--
作者：
Cao, Yu;Fan, Yang;Li, Fangyuan;Hao, Yu;Kong, Yaxian;Chen, Chen;Hao, Xing;Han, Dannuo;Li, Guoli;Wang, Zengtao;Song, Chuan;Han, Junyan;Zeng, Hui
背景单核细胞是炎症反应的重要介质之一，这一观点已被广泛接受。然而，它仍然不清楚是否老年单核细胞，类似于老年T细胞，具有超活化和增加的共抑制molecules.MethodsPeripheral blood mononuclear cells（PBMC）的表达的特征，分离自年轻人（21-40岁），中年人（41-60岁），和老年人受试者（> 60岁）。流式细胞术被用来监测单核细胞亚群和IFN-γ产生能力的表面分子的表达的变化。结果我们观察到增加肿瘤坏死因子-α：TNF-α和减少白细胞介素-6（IL-6）的产生，单核细胞从老年人相比，青年和中年人。老年人有更大比例的中间和非经典单核细胞亚群，沿着增加的免疫活化标志物人白细胞抗原-DR（HLA-DR），粘附分子分化分子簇11 b（CD 11 b）和L-选择素（CD 62 L）水平。此外，我们观察到在老年受试者中，经典单核细胞上C-C基序趋化因子受体2（CCR 2）表达增加，非经典单核细胞上C-X3-C基序趋化因子受体1（CX 3CR 1）表达减少。共抑制受体的表达减少单核细胞亚群在老年adults.ConclusionsCirculating单核细胞在老年人表现出增加的活化，粘附和迁移标志物的表达，但共抑制分子的表达减少。
BackgroundIt has been widely accepted that monocytes are one of the central mediators contributing to inflammaging. However, it remains unclear whether aged monocytes, similar to aged T cells, have characteristics of hyperactivation and increased expression of co-inhibitory molecules.MethodsPeripheral blood mononuclear cells (PBMCs) were isolated from young (21–40 years old), middle-aged (41–60 years old), and older human subjects (> 60 years old). Flow cytometry was used to monitor changes in the expression of surface molecules of monocyte subsets and cytokine-producing capacity.ResultsWe observed increased tumor necrosis factor-α: TNF-α and decreased interleukin-6 (IL-6) production in monocytes from older adults compared with young and middle-aged adults. Older adults had a greater percentage of intermediate and non-classical monocyte subsets, along with increased levels of the immune activation markers human leukocyte antigen-DR (HLA-DR), and adhesion molecules cluster of differentiation molecule 11b (CD11b) and L-selectin (CD62L). Furthermore, we observed increased C–C motif chemokine receptor 2 (CCR2) expression on classical monocytes and decreased C-X3-C motif chemokine receptor 1 (CX3CR1) expression on non-classical monocytes in older adult subjects. The expression of co-inhibitory receptors was reduced on monocyte subsets in older adults.ConclusionsCirculating monocytes in older adults exhibit increased expression of activation, adhesion, and migration markers, but decreased expression of co-inhibitory molecules.
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影响因子：
4.6
作者：
Fraser SD;Sadofsky LR;Kaye PM;Hart SP
通讯作者：
Hart SP
DOI：
10.1093/gerona/glv119
发表时间：
2016-09-01
影响因子：
5.1
作者：
Janssen, Nicole;Derhovanessian, Evelyna;Pawelec, Graham
通讯作者：
Pawelec, Graham
影响因子：
32.4
作者：
Barkaway A;Rolas L;Joulia R;Bodkin J;Lenn T;Owen-Woods C;Reglero-Real N;Stein M;Vázquez-Martínez L;Girbl T;Poston RN;Golding M;Saleeb RS;Thiriot A;von Andrian UH;Duchene J;Voisin MB;Bishop CL;Voehringer D;Roers A;Rot A;Lämmermann T;Nourshargh S
通讯作者：
Nourshargh S
影响因子：
16.8
作者：
Ingersoll MA;Platt AM;Potteaux S;Randolph GJ
通讯作者：
Randolph GJ
影响因子：
7.3
作者：
Mony JT;Schuchert MJ
通讯作者：
Schuchert MJ