Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit
Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit
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DOI：
10.1083/jcb.200708176
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发表时间：
2008-04-07
影响因子：
7.8
通讯作者：
Nishida, Eisuke
中科院分区：
文献类型：
--
作者：
Nakajima, Hiroyuki;Yonemura, Shigenobu;Nishida, Eisuke
Myt1最初被认为是有丝分裂的主引擎--CDc2(CDK1)的抑制激酶，与Wee1一起被认为是有丝分裂进入的负调节因子。在这项研究中，我们报告了一个意想不到的发现，Myt1对于哺乳动物细胞末期高尔基体和内质网(ER)的组装是必不可少的。我们的分析表明，Cyclin B1和Cyclin B2都是Myt1的靶标，可以发生适当的高尔基体和内质网组装。因此，我们的结果表明，Myt1介导的对CDc2活性的抑制对于调控广泛的有丝分裂事件并不是必不可少的，但对于控制有丝分裂过程中的细胞膜动力学是特别必要的。
Myt1 was originally identified as an inhibitory kinase for Cdc2 (Cdk1), the master engine of mitosis, and has been thought to function, together with Wee1, as a negative regulator of mitotic entry. In this study, we report an unexpected finding that Myt1 is essential for Golgi and endoplasmic reticulum (ER) assembly during telophase in mammalian cells. Our analyses reveal that both cyclin B1 and cyclin B2 serve as targets of Myt1 for proper Golgi and ER assembly to occur. Thus, our results show that Myt1-mediated suppression of Cdc2 activity is not indispensable for the regulation of a broad range of mitotic events but is specifically required for the control of intracellular membrane dynamics during mitosis.