Association of CYP2C8, CYP2C9 and CYP2J2 gene polymorphisms with myocardial infarction in South Indian population
Association of CYP2C8, CYP2C9 and CYP2J2 gene polymorphisms with myocardial infarction in South Indian population
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DOI：
10.1016/j.pharep.2014.08.010
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发表时间：
2015-02-01
影响因子：
4.4
通讯作者：
Adithan, Chandrasekaran
中科院分区：
文献类型：
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作者：
Kumar, Annan Sudarsan Arun;Kumar, Srinivasamurthy Suresh;Adithan, Chandrasekaran
背景：心血管疾病（CVD）是全球死亡和发病的主要原因。心肌梗死（MI）是一种复杂的多因素、多基因疾病，由个体的遗传组成和各种环境因素之间的相互作用引起。CYP 2C 8、CYP 2C 9和CYP 2 J2基因参与花生四烯酸的代谢，产生环氧二十碳三烯酸（E3），介导冠状动脉扩张，改善缺血后心脏收缩功能，减轻血管炎症，增加血管内纤维蛋白溶解。这项研究的目的是分析CYP 2C 8，CYP 2C 9和CYP 2 J2基因多态性和MI risk in the South Indian population.Methods：这项回顾性研究包括287例MI患者，279例风险对照患者和321例健康人。采集所有受试者的血样，采用标准酚-氯仿法提取DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和真实的时间-聚合酶链反应（RT-PCR）方法进行基因分型。要测试潜在的独立关联之间的多态性和MI的风险，多元Logistic回归分析performed.Results：我们的研究结果显示，CYP 2 J2 *7（p = 0.04; OR = 2.0）多态性和MI之间的显着关联，而比较的情况下，以风险控制。我们没有观察到任何关联的CYP 2C 8 *2，CYP 2C 8 *3，CYP 2C 9 *2和CYP 2C 9 *3与MI。结论：我们的研究结果表明，任何传统的MI危险因素与CYP 2 J2 *7变异等位基因沿着的个人可能倾向于在南印度人口的MI的风险。(C)2014年波兰科学院药理学研究所。出版社：Elsevier Urban & Partner Sp. Z o.o. All rights reserved.
Background: Cardiovascular diseases (CVDs) are the major cause of mortality and morbidity worldwide. Myocardial infarction (MI) is a complex multi-factorial, polygenic disorder arising from an interaction between genetic makeup of individuals and various environmental factors. CYP2C8, CYP2C9 and CYP2J2 gene involved in the metabolism of arachidonic acid, generates epoxyeicosatrienoic acids (EETs) that mediate dilation of coronary arteries improving post-ischemic cardiac contractile function, reduce vascular inflammation, and increase intravascular fibrinolysis. The study is aimed at analyzing the association of CYP2C8, CYP2C9 and CYP2J2 gene polymorphisms and MI risk in the South Indian population.Methods: This retrospective study consisted of 287 MI patients, 279 risk control patients and 321 healthy individuals. Blood samples were collected from all the subjects and DNA was isolated using standard phenol-chloroform method. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and real time-polymerase chain reaction (RT-PCR) methods were used for genotyping. To test the potential independent association between polymorphisms and the risk of MI, Multiple-logistic regression analysis was performed.Results: Our findings displayed a significant association between CYP2J2*7 (p = 0.04; OR = 2.0) polymorphism and MI while comparing cases with to risk controls. We did not observe any association of CYP2C8*2, CYP2C8*3, CYP2C9*2 and CYP2C9*3 with MI.Conclusion: Our results suggest that individuals with any conventional risk factor for MI along with CYP2J2*7 variant allele may be predisposed to risk of MI in South Indian population. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. Z o.o. All rights reserved.