Mycobacterium tuberculosis isolates grown under oxygen deprivation invade pulmonary epithelial cells
Mycobacterium tuberculosis isolates grown under oxygen deprivation invade pulmonary epithelial cells
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DOI：
10.1016/j.anaerobe.2012.04.010
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发表时间：
2012-08-01
期刊：
影响因子：
2.3
通讯作者：
Sturm, A. Willem
中科院分区：
文献类型：
--
作者：
Ashiru, Olubisi T.;Pillay, Manormoney;Sturm, A. Willem
结核分枝杆菌具有适应和在不同环境条件下生存的能力，包括缺氧。 To better understand the pathogenesis of M. tuberculosis, we studied the invasion of human alveolar (A549) and human bronchial (BBM) epithelial cell lines by M. tuberculosis isolates cultured under oxygen deprivation.我们使用属于北京和 F15/1AM4/KZN 家族的分离株、具有独特 DNA 指纹的分离株以及实验室菌株 H37Rv 和 H37Ra。我们确定：（1）缺氧条件下生长的结核分枝杆菌侵入上皮细胞，（2）所有17个分离株的侵袭能力不同，（3）缺氧影响这些分离株的侵袭能力。所有分离株比 BBM 细胞更有效地侵入 A549。三种 F15/LAM4/KZN 分离株（其中两种具有广泛耐药性 (XDR)）的侵袭性 (>= 33%) 至少是侵袭性最强的北京分离株 (15%) 的两倍 (P < 0.05)。我们的结论是，为了更全面地了解结核分枝杆菌的发病机制，研究应包括在缺氧条件下培养的分离株。 (C) 2012 Elsevier Ltd. 保留所有权利。
Mycobacterium tuberculosis has the ability to adapt to and survive under different environmental conditions, including oxygen deprivation. To better understand the pathogenesis of M. tuberculosis, we studied the invasion of human alveolar (A549) and human bronchial (BBM) epithelial cell lines by M. tuberculosis isolates cultured under oxygen deprivation. We used isolates belonging to the Beijing and F15/1AM4/KZN families, isolates with unique DNA fingerprints and the laboratory strains H37Rv and H37Ra. We determined that: (1) M. tuberculosis bacilli grown under oxygen deprivation invade epithelial cells, (2) the invasion capacity of all 17 isolates differed, and (3) oxygen deprivation influenced the invasion capacity of these isolates. All isolates invaded the A549 more effectively than the BBM cells. Three of the F15/LAM4/KZN isolates, two of which had extensively drug resistance (XDR) profiles, were at least twice as invasive (>= 33%) as the most invasive Beijing isolate (15%) (P < 0.05). We conclude that for a more comprehensive understanding of the pathogenesis of M. tuberculosis, studies should include isolates that have been cultured under oxygen deprivation. (C) 2012 Elsevier Ltd. All rights reserved.