Xenophilus arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from soil.
Xenophilus arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from soil.
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DOI：
10.1099/ijs.0.061515-0
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发表时间：
2014-06
影响因子：
2.8
通讯作者：
Qin-fen Li;Li-na Sun;Soon-Wo Kwon;Qing Chen;Jian He;Shun-Peng Li;Jun Zhang
中科院分区：
文献类型：
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作者：
Qin-fen Li;Li-na Sun;Soon-Wo Kwon;Qing Chen;Jian He;Shun-Peng Li;Jun Zhang
从农田土壤中分离到一株革兰氏阴性、需氧、运动、杆状、抗砷的细菌YW 8（T）。16 S rRNA基因序列分析显示，与环境物种Xenophilus azovorans，Xenophilus aerolatus，Simplicispira metamorpha，Variovorax soli和Xylophilus ampelinus的菌株的序列相似性超过97%。系统发育树分析表明YW 8（T）菌株与嗜氮嗜血杆菌形成了独立的进化枝。DNA-DNA杂交结果表明，菌株YW 8（T）与其亲缘关系最近的几个种之间的DNA-DNA相关度在24.2- 35.5%之间，将菌株YW 8（T）与亲缘关系最近的几个种区分开来。菌株YW 8（T）的主要细胞脂肪酸为C（16：0）、C（17：0）cyclo、C（18：1）ω 7 c和总特征3（C（16：1）ω6c和/或C（16：1）ω 7 c）。基因组DNA G+C含量为69.3mol%，主要呼吸醌是泛醌-8。主要极性脂质为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、三种未知磷脂、一种未知极性脂质和磷脂酰丝氨酸。主要的多胺是2-羟基腐胺和腐胺。根据形态特征、生理生化特征、系统发育位置、DNA-DNA杂交和化学分类学资料，认为菌株YW 8（T）代表Xenophilus属的一个新种，并建议将其命名为Xenophilus arsenicistens sp. nov.，模式菌株为YW 8（T）（= CCTCC AB 2012103（T）= KACC 16853（T））。
A Gram-reaction-negative, aerobic, motile, rod-shaped, arsenite [As(III)]-resistant bacterium, designated strain YW8(T), was isolated from agricultural soil. 16S rRNA gene sequence analysis showed over 97% sequence similarity to strains of the environmental species Xenophilus azovorans, Xenophilus aerolatus, Simplicispira metamorpha, Variovorax soli, and Xylophilus ampelinus. However, the phylogenetic tree indicated that strain YW8(T) formed a separate clade from Xenophilus azovorans. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness values between strain YW8(T) and its closest phylogenetic neighbours were below 24.2-35.5%, which clearly separated the strain from these closely related species. The major cellular fatty acids of strain YW8(T) were C(16 : 0), C(17 : 0) cyclo, C(18 : 1)ω7c, and summed feature 3(C(16 : 1)ω6c and/or C(16 : 1)ω7c). The genomic DNA G+C content was 69.3 mol%, and the major respiratory quinone was ubiquinone-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, an unknown polar lipid and phosphatidylserine. The major polyamines were 2-hydroxyputrescine and putrescine. On the basis of morphological, physiological and biochemical characteristics, phylogenetic position, DNA-DNA hybridization and chemotaxonomic data, strain YW8(T) is considered to represent a novel species of the genus Xenophilus, for which the name Xenophilus arseniciresistens sp. nov. is proposed; the type strain is YW8(T) ( = CCTCC AB2012103(T) = KACC 16853(T)).