Pangenome of Serratia marcescens strains from nosocomial and environmental origins reveals different populations and the links between them
Pangenome of Serratia marcescens strains from nosocomial and environmental origins reveals different populations and the links between them
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DOI：
10.1038/s41598-018-37118-0
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发表时间：
2019-01-10
影响因子：
4.6
通讯作者：
Altier, Nora
中科院分区：
文献类型：
--
作者：
Abreo, Eduardo;Altier, Nora
粘质沙雷氏菌（Serratia marcescens）是一种革兰氏阴性细菌，可在土壤、水和植物表面等广泛的环境中发现，同时它也被称为医院中的机会性人类病原体和作物中的植物生长促进细菌（PGPR）。我们已经使用了一个泛基因组为基础的方法，基于公开的基因组，应用全基因组多位点序列类型计划，以评估是否有一个源和基因型之间的关联，旨在区分分离株从医院来源和环境，和菌株之间的报告为PGPR从其他环境菌株。来自医院环境和环境来源的大多数基因组可以被分配到拟议的医院或环境MLST，这表明来源和基因型之间的关联。来自医院来源的少数基因组显示环境MLST的事实表明，少数医院菌株最近来自环境。PGPR菌株被分配到不同的环境类型和进化枝，但只有一个沼泽包括积累了少量已知的毒力和抗生素抗性决定簇的菌株，并完全来自环境来源。这片林地被设想为一组有前途的MLST，用于选择潜在的PGPR菌株。
Serratia marcescens is a Gram-negative bacterial species that can be found in a wide range of environments like soil, water and plant surfaces, while it is also known as an opportunistic human pathogen in hospitals and as a plant growth promoting bacteria (PGPR) in crops. We have used a pangenome-based approach, based on publicly available genomes, to apply whole genome multilocus sequence type schemes to assess whether there is an association between source and genotype, aiming at differentiating between isolates from nosocomial sources and the environment, and between strains reported as PGPR from other environmental strains. Most genomes from a nosocomial setting and environmental origin could be assigned to the proposed nosocomial or environmental MLSTs, which is indicative of an association between source and genotype. The fact that a few genomes from a nosocomial source showed an environmental MLST suggests that a minority of nosocomial strains have recently derived from the environment. PGPR strains were assigned to different environmental types and clades but only one Glade comprised strains accumulating a low number of known virulence and antibiotic resistance determinants and was exclusively from environmental sources. This Glade is envisaged as a group of promissory MLSTs for selecting prospective PGPR strains.