Transcriptional variation analysis of Arabidopsis ecotypes in response to drought and salt stresses dissects commonly regulated networks
Transcriptional variation analysis of Arabidopsis ecotypes in response to drought and salt stresses dissects commonly regulated networks
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拟南芥生态型响应干旱和盐胁迫的转录变异分析剖析了共同调控的网络
DOI：
10.1111/ppl.13295
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发表时间：
2021
影响因子：
6.4
通讯作者：
Zhulong Chan
中科院分区：
文献类型：
--
作者：
Yanping Wang;Zhengfu Fang;Li Yang;Zhulong Chan
盐度和干旱条件通常会导致渗透和氧化应激，而盐度还会导致离子应激。在这项研究中，我们鉴定了五种拟南芥生态型中受渗透和离子胁迫调节的特定基因。 Shahdara (SHA) 和 C24 生态型在幼苗生长阶段对盐和干旱胁迫具有更强的耐受性，与其他三种生态型相比，在干旱和盐分条件下表现出较低的失水率、较低的电解质泄漏和较高的成活率。转录组分析显示，盐胁迫和渗透胁迫分别对 3700 个和 2242 个基因进行差异调节。渗透胁迫上调基因的 78.1% 和下调基因的 62.0% 也普遍受到盐胁迫的调节。基因本体术语富集分析表明，生长素吲哚-3-乙酸（IAA）、脱落酸、细胞分裂素和赤霉酸途径受渗透胁迫调节，而IAA、茉莉酸和乙烯途径受离子胁迫改变。营养物和水吸收途径受到渗透压和离子应激的调节，而离子运输和激酶途径则受到离子应激的调节。此外，我们将 bHLH61 描述为响应盐和干旱胁迫的负调节因子。这项研究为植物对盐和干旱胁迫的反应提供了新线索。
Salinity and drought conditions commonly result in osmotic and oxidative stresses, while salinity additionally causes ionic stress. In this study, we identified specific genes regulated by osmotic and ionic stresses in five Arabidopsis ecotypes. Shahdara (SHA) and C24 ecotypes were more tolerant to salt and drought stresses at the seedling growth stage, as evidenced by lower water loss rate, lower electrolyte leakage, and higher survival rate when compared to the other three ecotypes under drought and salinity conditions. Transcriptomic analysis revealed that 3700 and 2242 genes were differentially regulated by salt and osmotic stresses, respectively. Totally 78.1% of upregulated and 62.0% of downregulated genes by osmotic stress were also commonly regulated by salt stress. Gene ontology term enrichment analysis showed that auxin indole‐3‐acetic acid (IAA), abscisic acid, cytokinin, and gibberellic acid pathways were regulated by the osmotic stress, while IAA, jasmonic acid, and ethylene pathways were changed by the ionic stress. The nutrient and water uptake pathways were regulated by both the osmotic and ionic stresses, whereas ion transportation and kinase pathways were modulated by the ionic stress. Additionally, we characterizedbHLH61as a negative regulator in response to salt and drought stresses. This study provided new clues of plant responses to salt and drought stresses.