In vivo effect of industrial titanium dioxide nanoparticles experimentally exposed to hairless rat skin
In vivo effect of industrial titanium dioxide nanoparticles experimentally exposed to hairless rat skin
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DOI：
10.3109/17435391003793095
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发表时间：
2010-09-01
期刊：
影响因子：
5
通讯作者：
Yamamoto, Osamu
中科院分区：
文献类型：
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作者：
Adachi, Koji;Yamada, Nanako;Yamamoto, Osamu
我们形态学研究动物皮肤暴露于W/O乳液含有10重量%的超细TiO 2颗粒，其特征在于。4 h后，通过光学显微镜、共聚焦激光扫描显微镜（CLSM）和电子显微镜结合能量色散X射线光谱法（EDX）对暴露皮肤进行研究。还在24、72和168小时后对暴露的皮肤进行了光学显微镜评价。光学显微镜未显示皮肤的任何形态学和免疫组织化学变化。电子显微镜观察发现，大多数TiO 2颗粒定位于滤泡间分离层和滤泡漏斗角化层。在活皮肤中未检测到TiO 2颗粒，这通过EDX证实。此外，我们通过光学显微镜和低真空扫描电子显微镜与EDX证明了特定的TiO 2对毛囊开口区域的亲和力。我们的研究表明，TiO 2颗粒既不会渗透到活细胞层中，也不会在生物学上引起任何细胞变化。
We morphologically investigated animal skin exposed to W/O emulsion containing 10 wt % ultrafine TiO2 particles that had been characterized. After 4 h, exposed skin was investigated by light microscopy, confocal laser scanning microscopy (CLSM) and electron microscopy with energy-dispersive X-ray spectrometry (EDX). Light microscopic evaluation was also performed on the exposed skin after 24, 72 and 168 h. Light microscopy did not show any morphological and immunohistochemical changes in the skin. Electron microscopy revealed that the most TiO2 particles were localized in the interfollicular stratum disjunctum and the keratinized layer of follicular infundibulum. No TiO2 particles were detected in the viable skin, which was confirmed by EDX. Furthermore, we demonstrated a specific TiO2 affinity to the follicular opening area by light microscopy and low-vacuum scanning electron microscopy with EDX. Our study suggests that TiO2 particles neither penetrate into viable cell layers nor biologically cause any cellular changes.