In vitro- and in vivo-induced transgene expression in human embryonic stem cells and derivatives
In vitro- and in vivo-induced transgene expression in human embryonic stem cells and derivatives
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DOI：
10.1634/stemcells.2007-0710
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发表时间：
2008-02-01
期刊：
影响因子：
5.2
通讯作者：
Zhang, Su-Chun
中科院分区：
文献类型：
--
作者：
Xia, Xiaofeng;Ayala, Melvin;Zhang, Su-Chun
条件基因表达将促进人类胚胎干细胞（hESC）作为研究和治疗工具的使用。在这里，我们报告了药物诱导的转基因表达，在体外和体内，从一个tet-on人胚胎干细胞系与>95%的纯度。使用绿色荧光蛋白作为指示剂，我们证明tet-on系统允许在未分化的hESC和三个胚层的分化细胞中的基因表达的严格控制。更重要的是，在细胞移植到动物体内后，基因表达仍然受到口服药物的调节。这些结果为hESC及其衍生物的基因表达调控提供了技术基础。
The use of human embryonic stem cells (hESCs) as a research and therapeutic tool will be facilitated by conditional gene expression. Here, we report drug-induced transgene expression, both in vitro and in vivo, from a tet-on hESC line with >95% purity. Using green fluorescent protein as an indicator, we demonstrated that the tet-on system allowed a tight control of the gene expression in both undifferentiated hESCs and differentiated cells of the three germ layers. More importantly, after the cells were transplanted into animals, the gene expression remained to be regulated by an orally administered drug. These results provide a technical basis for regulation of gene expression in hESCs and derivatives in vitro and in vivo.