Overexpression of soybean GmPLDγ enhances seed oil content and modulates fatty acid composition in transgenic Arabidopsis
Overexpression of soybean GmPLDγ enhances seed oil content and modulates fatty acid composition in transgenic Arabidopsis
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大豆 GmPLDγ 的过度表达可提高转基因拟南芥种子油含量并调节脂肪酸组成
DOI：
10.1016/j.plantsci.2019.110298
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发表时间：
2020-01-01
期刊：
影响因子：
5.2
通讯作者：
Zhang, Wenhua
中科院分区：
文献类型：
--
作者：
Bai, Yang;Jing, Guangqin;Zhang, Wenhua
磷脂酶 D (PLD) 水解甘油磷脂的磷酸二酯键，产生磷脂酸 (PA) 和游离头基。 PLD 对于植物生长、发育和对外部胁迫的反应很重要。然而，它们在三酰甘油（TAG）合成中的作用仍不清楚。在这里，我们报道了大豆 (Glycine max) PLD gamma (GmPLD gamma) 参与甘油脂周转和种子油生产。 GmPLD gamma 靶向线粒体并表现出由油酸和磷脂酰肌醇 4,5-二磷酸 [PtdIns(4,5)P-2] 激活的 PLD 活性。在拟南芥中过度表达 GmPLD gamma（缩写为 GmPLD gamma-OE）会导致种子重量增加，18、20 和 22 碳脂肪酸 (FA) 的 TAG 水平升高，并改变油体形态。此外，营养组织中的膜脂水平显着降低，而成熟种子中除了 GmPLD γ-OE 品系中的二半乳糖基二酰基甘油 (DGDG) 水平降低外，没有发现明显的变化。此外，在 GmPLD gamma-OE 品系中发育的长角果中，参与甘油脂代谢的基因表达显着上调。总之，我们的数据表明 GmPLD γ 在 TAG 合成和脂肪酸重塑中的调节作用，强调了线粒体定向的甘油磷脂稳态在种子油积累中的重要性。
Phospholipase D (PLD) hydrolyzes the phosphodiester bond of glycerophospholipids to yield phosphatidic acid (PA) and a free headgroup. PLDs are important for plant growth, development, and responses to external stresses. However, their roles in triacylglycerol (TAG) synthesis are still unclear. Here, we report that a soybean (Glycine max) PLD gamma (GmPLD gamma) is involved in glycerolipid turnover and seed oil production. GmPLD gamma was targeted to mitochondria and exhibited PLD activity that was activated by oleate and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P-2]. Overexpression of GmPLD gamma (abbreviated GmPLD gamma-OE) in Arabidopsis thaliana resulted in enhanced seed weight, elevated levels of TAGs with 18-, 20-, and 22-carbon fatty acids (FAs), and altered oil-body morphology. Furthermore, the levels of membrane lipids in vegetative tissues decreased significantly, whereas no overt changes were found in mature seeds except for a decrease in the digalactosyldiacylglycerol (DGDG) level in the GmPLD gamma-OE lines. Additionally, the expression of genes involved in glycerolipid metabolism was significantly upregulated in developing siliques in GmPLD gamma-OE lines. Together, our data indicate a regulatory role for GmPLD gamma in TAG synthesis and fatty-acid remodeling, highlighting the importance of mitochondria-directed glycerophospholipid homeostasis in seed oil accumulation.