In vivo generation of dental pulp-like tissue by using dental pulp stem cells, dentin matrix protein 1 transplantation in mice
In vivo generation of dental pulp-like tissue by using dental pulp stem cells, dentin matrix protein 1 transplantation in mice
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DOI：
10.1016/j.joen.2008.02.005
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发表时间：
2008-04-01
影响因子：
4.2
通讯作者：
George, Anne
中科院分区：
文献类型：
--
作者：
Prescott, Rebecca S.;Alsanea, Rajaa;George, Anne
穿孔的存在是众所周知的显着妥协的结果根管治疗。再生根管治疗的一个潜在用途可能是修复根管穿孔。除了营养素和全身原位相互作用外，认为对组织再生至关重要的3种主要成分是干细胞，支架和生长因子。本研究采用小鼠模型，观察了组织工程三联体中各组分在牙髓干细胞（DPSC）在模拟分叉穿孔部位的组织和分化中的作用。胶原作为支架，牙本质基质蛋白1（DMP1）是生长因子。将材料放置在牙本质切片中的模拟穿孔部位。三氧化物矿物质聚集体是对照修复材料。6周后处死动物，通过光学显微镜和组织学染色评价穿孔部位。在含有DPSC、胶原支架和DMP1三联体的组中观察到新衍生的牙髓组织的组织。其他4组未表现出任何明显的组织结构。在本研究的条件下，可以得出结论，DPSC、胶原支架和DMP1的三联体可以诱导类似于牙髓组织的组织化基质形成，这可能导致硬组织形成。
The presence of a perforation is known to significantly compromise the outcome of endodontic treatment. One potential use of regenerative endodontic therapy might be the repair of root canal perforations. In addition to nutrients and systemic in situ interactions, the 3 main components believed to be essential for tissue regeneration are stem cells, scaffold, and growth factors. This study investigated the role of each component of the tissue engineering triad in the organization and differentiation of dental pulp stem cells (DPSCs) in a simulated furcal perforation site by using a mouse model. Collagen served as the scaffold, and dentin matrix protein 1 (DMP1) was the growth factor. Materials were placed in simulated perforation sites in dentin slices. Mineral trioxide aggregate was the control repair material. At 6 weeks, the animals were killed, and the perforation sites were evaluated by light microscopy and histologic staining. Organization of newly derived pulp tissue was seen in the group containing the triad of DPSCs, a collagen scaffold, and DMP1. The other 4 groups did not demonstrate any apparent tissue organization. Under the conditions of the present study, it might be concluded that the triad of DPSCs, a collagen scaffold, and DMP1 can induce an organized matrix formation similar to that of pulpal tissue, which might lead to hard tissue formation.